Autosomal DNA Tests for Genealogy

First a disclaimer.  I'm very new to the whole world of genetic genealogy.  I'm not new however, to traditional genealogy, and I do have a pretty good amateur understanding of relative archaeological and anthropological discussions over the past fifty years.  The following is not meant as a critique of genetic genealogy, so much as a review, or my experience, of ancestry composition based on autosomal DNA analysis.

Let's start with my paper trail.

Traditional Genealogy

I am English by ethnicity, British by nationality, and a subject of Queen Elizabeth II (often now referred to as a UK Citizen).

My paper recorded ancestry consists of the genealogical records of:

  • Generation 1 has 1 individual. (100.00%)
  • Generation 2 has 2 individuals. (100.00%)
  • Generation 3 has 4 individuals. (100.00%)
  • Generation 4 has 8 individuals. (100.00%)
  • Generation 5 has 16 individuals. (100.00%)
  • Generation 6 has 29 individuals. (90.62%)
  • Generation 7 has 49 individuals. (76.56%)
  • Generation 8 has 35 individuals. (27.34%)
  • Generation 9 has 24 individuals. (10.16%)
  • Generation 10 has 10 individuals. (2.34%)
  • Generation 11 has 4 individuals. (0.39%)
  • Total ancestors in generations 2 to 11 is 181. (9.04%)

All 181 ancestors, reaching back to the 1690's, appear to be English born, of English ethnicity, with English surnames.  The majority of them (100% on my mother's side, and 81% on my father's side) were East Anglian, with the vast majority of that percentage being born in the county of Norfolk.  Religions recorded or indicated were CofE Anglican or non-conformist Christian.  No sign of any Catholicism, Islam, or Judaism.

Therefore it would look pretty likely, that I can claim English heritage, wouldn't you agree?

Genetic Genealogy and Ancestry Prediction

There are three aspects or avenues of inquiry, available for genetic genealogy.  First of all, the two sex haplogroups; the y-DNA, and the mt-DNA. These two "signals" are referred to as haplogroups.

  1. The y-DNA.  This follows the Y chromosome.  It is only carried by men.  It is passed along the paternal line, and only by that line, from grandfather, down to father, down to son, until the line is broken.  What a lot of people do often misunderstand, is that it does not represent 50% of your ancestry.  It does not represent all of your biological father's ancestry.  For example, his mother's father, and her brothers, although on your father's side, would most likely carry a different y-DNA haplogroup.  It only comes down an uninterrupted strictly paternal line.  Even at Generation 7 (g.g.g.g grandparents) above, it would have been carried by one out of my sixty four biological ancestors at that generation.  The other thirty one g.g.g.g grandfathers for that generation may have carried different Y haplogroups.
  2. The mt-DNA.  Although a very different type of DNA, this one works as the opposite sex haplogroup.  It is a signal that is passed down the strictly maternal line, from grandmother, to mother, to her children.  Yes, we men do inherit our mother's mt_DNA, but we can't pass it down.  Only our sisters can.
  3. The au-DNA, better known as Autosomal DNA.  Whereas the former two sex haplogroups are handy, because we can measure their mutations, and track their formation and movement across thousands of years, au-DNA really is the stuff that we are made of - all of the SNPs on our chromosomes that personalise us within the human genome.  We inherit our au-DNA from all of our recent ancestors.  Roughly 50% from our biological mother, and 50% from our biological father.  Equally, we could say on average, 25% from each grandparent, or 12.5% from each great grandparent.  However, it is messy.  At every reproduction (meiosis), it gets messed up by recombination.  Not only that, but go back much more than six generations, and it becomes more and more likely that you can lose entire lineages.  You can have no surviving trace of any DNA from for example, a particular g.g.g.g.g grandparent.

Autosomal DNA is what makes us individuals, gives us our hereditary traits.  It is passed down from many ancestors, via our parents.  However, the sex haplogroups are of interest because they can be traced across the globe, and the millennia.  As we gain more and more data - both from living populations, and ancient DNA from archaeological finds, so we will be able to track the STR and SNP mutation data more precisely.

However, what about poor old messed up autosomal DNA?  It represents our entire biological heritage over many generations. It is what we are. However, making sense of it is less easy, less precise.  Genetic genealogists are making progress, but it is far less of a precise science than either of the haplogroups.  They use calculators, that measure the segments of DNA cross the chromosomes, looking for patterns that they recognise from a number of known reference populations.  From that, these calculators predict an ancestry.  Exactly what and when that ancestry refers to, does seem to vary from one calculator to another.  There is an argument that the precision can be improved if you also test close known relatives including at least one parent.  The results can then be phased.  I'm actually waiting for the results for my mother, so that I can see my own au-DNA ancestry results phased and corrected.

So lets have a bit of fun, and see what some of the calculators suggest for my autosomal DNA, at least before any phasing with my mother's DNA.  What do they make of my 100% English paper ancestry?

23andMe.com Ancestry Composition Standard Mode

99.9% European.

Broken into:

83% NW European

17% Broadly (unassigned) European

I think that's pretty cool.  As I'm getting to know au-DNA predictions, so as I'm learning to appreciate it when they get the right continent, and the right corner of that continent.  That is more than they could do a decade or two ago.  The prediction is correct, I am a NW European.  I'm not a West African, a South Asian, or a East Siberian.

23andMe.com Ancestry Composition Speculative Mode

100% European

Broken into:

94% NW European

3% S European

3% Broadly (unassigned) European.

Whoa, where did that South European come from?  It could just be a stray incorrectly identified signal, or it could be telling me that one of my ancestors, maybe around Generation 6, were from down south!  Lets break down the prediction further.  First, the NW European:

32% British & Irish

27% French & German

7% Scandinavian

But surely I should be 100% British & Irish?  Not only 32%.  I have my own ideas about this.  I think that although 23andMe claims that Ancestry Composition only represents the ancestry of the past 300 to 500 years (the so-called migration period, as sold to USA customers), that it gets confused by earlier migrations across their reference populations, including those during the early medieval period, and perhaps even some of those during late prehistory.  I've noticed that across Ireland and Britain, the further to the east, the more diluted the 23andMe British & Irish assignment.  People of solid Irish ancestry get between 85% and 98% British & Irish.  My East Anglian results, mixed between British & Irish, French & German, and Scandinavian, are actually rather more like those received by Dutch customers of 23andMe.

As for that Southern European prediction, how does that break down?

0.5% Iberian

2.4% Broadly (unassigned) South European.

Which if taken seriously, might suggest that I have an unknown Spanish or Portuguese ancestor around Generation 6.  If I did take it seriously that is.  I wonder what my mother's test will reveal?

DNA.Land.com Ancestry Composition

This is a third party site, that you can upload your 23andMe V4 raw data to, and see what their calculators predict for your ancestry.  It has recently had it's ancestry composition revised.  What did that make of my 100% English au-DNA?

West Eurasian 100%.

I like that designation, the amateur anthropologist in me prefers that broad designation over "European".  Broken down:

77% North/Central European

19% South European

2.4% Finnish

1.3% unassigned.

What?  Why not 100% North/Central European?  Finnish?  Did some early medieval Scandinavian settlers of East Anglia bring it?  Or is it a false signal?  Misidentified au-DNA?

That darned South European kicked in again.  I'm here looking at a biological cuckoo NPE (non-parental event) at around Generation 5 or even more recent!  Did a great grandmother secretly have a South European lover?  But this South European breaks down further:

13% Balkan

6% Italian.

Oh my goodness, whereas 23andMe speculative mode suggested SW Europe - this one suggests SE Europe!  Do I have a secret Albanian great grandfather?  Or is it all nonsense?

WeGene.com

This is a cracking new third party DNA analyser.  It is based in China, and it's predictors appear to calculate mainly for a Chinese market.  It not only predicts your ancestry composition, but also your two sex haplogroups, and lots of traits and health predictions to compliment those of 23andMe.  It even tries to predict your genetic disposition to sexuality!

It will allow you to send your 23andMe V4 raw data direct to it's own calculators.  However, at the moment the website is almost entirely in Chinese (Mandarin?).  There are two options.  1) At the bottom of the webpages is a hyperlink to English, which gives, in English, a basic ancestry composition, and your haplogroups.  It does not include English versions of the health and trait results.  2) use an online translator, such as the one built into the Google Chrome browser.  It actually serves pretty well.

On sex haplogroups they give my Y-DNA as

L1.  Not bad, but they didn't make it to L1b or L-M317.

My mtDNA?

H6a1a8.  Very good.  Better than 23andMe's H6a1, and the same as the mthap program.

But this is about au-DNA, how did they do, what did they make of my 100% English ancestry?

81% French

19% English/Briton

Now, that sounds pretty awful, but on closer inspection, I'm impressed.  No South European great grandfather.  Okay, so most of my DNA has been placed on the wrong side of the Channel.  However, I know that French and English DNA is actually very close.  Recent surveys even suggest that the English have inherited a lot of common ancestry with the French during unknown migration late in prehistory.  So again - they very much got the right corner of the right Continent.  Well done WeGene.

GEDmatch.com Eurogenes K13

GEDmatch is a website that you can upload raw data not only from 23andMe, but from a range of testers, and from V3 chips as well as V4.  It hosts a number of tools and predictors - some Open Source.  Some of these predictors are for Admixture or ancestry composition.  They measure your ancestry in terms of distance from known reference populations.  The lower the number, the closer you are to their reference.  They use calculators known as oracles to predict ancestry, including mixed ancestry or admixture.

The oracles on the Eurogenes K13 and K15 calculator models have a good reputation at working with West Eurasian ancestry.  So how does K13 first, score my 100% English ancestry?

On Single Population Sharing, it rates my DNA against the closest references.  In order of closest to not so close, the top five are:

1 South_Dutch 3.89
2 Southeast_English 4.35
3 West_German 5.22
4 Southwest_English 6.24
5 Orcadian 6.97

I think that's a cracking result.  Okay, it thinks that I'm closer to South Dutch, than I am to SE English, but so close - and my East Anglian ancestry most likely does include a lot of admixture from the Low Countries from the early medieval period.  I really like Eurogenes K13.

Okay, let's now use the Oracle 4 option, to suggest admixture.  First on three populations admixing to create my DNA, what comes closest?

50% Southeast_English +25% Spanish_Valencia +25% Swedish @ 2.087456

Well that's interesting!  The SE English hit the net.  The Swedish?  Could be ancient Scandinavian admixture - but the Iberian prediction has reemerged!

On four populations admixing?

1 Southeast_English + Southeast_English + Spanish_Valencia + Swedish @ 2.087456
2 Southeast_English + Southeast_English + Spanish_Murcia + Swedish @ 2.147237
3 Norwegian + Portuguese + Southeast_English + Southeast_English @ 2.216714
4 Danish + Portuguese + Southeast_English + Southeast_English @ 2.225334
5 Portuguese + Southeast_English + Southeast_English + Swedish @ 2.230991

Oh my goodness.  K13 agrees with 23andMe AC, that I have an Iberian link.  I'm now really starting to wonder.

Let's finish off by trying K15 on my 100% English ancestry:

GEDmatch.com Eurogenes EU test V2 K15


Using Oracle for single population first, the top five closest:

1 Southwest_English 2.7
2 South_Dutch 3.98
3 Southeast_English 4.33
4 Irish 6.23
5 West_German 6.25

Okay, I'm SE English, not SW English, but pretty impressive again.

Using the oracle 4 for three population admixture, what mix comes closest to my auDNA?

50% Southwest_English +25% Spanish_Castilla_Y_Leon +25% West_Norwegian @ 1.080952

That Iberian back again!

Top five mix ups of populations closest to me?

1 Southwest_English + Southwest_English + Spanish_Castilla_Y_Leon + West_Norwegian @ 1.080952
2 Irish + North_Dutch + Southwest_English + Spanish_Galicia @ 1.111268
3 North_Dutch + Southwest_English + Spanish_Galicia + West_Scottish @ 1.282744
4 Southeast_English + Southwest_English + Spanish_Castilla_Y_Leon + West_Norwegian @ 1.295819
5 North_Dutch + North_Dutch + Southwest_English + Spanish_Castilla_Y_Leon @ 1.304939

I can't help preferring the K13 results to the EU test V2 K15 - simply because it recognises me better as SE English, rather than to their SW English reference.

Conclusions

If anyone ever bothers reading this far too lengthy post, I hope that I have imparted the following lessons:

  • Don't expect DNA Ancestry tests to pin point an actual country of ancestry.  They're not no where near that good yet.  The populations of West Eurasia, and elsewhere, are actually all mixed up, or share a lot of recent admixture.  In addition, many European nation-states are quite recent inventions.  I've seen the borders of Europe change in my short lifetime.
  • Don't expect precision.  If for example, you are an American, and a 23andMe AC test suggests only 32% British & Irish, then you could actually have 100% English ancestry over the past 300 years!  We're so mixed up, that these tests are struggling to part and identify us by nationality.
  • If you are willing to share your raw data (there are privacy issues), then have fun trying out all of these third party calculators.  It's a lot of fun as you can see.  They rarely agree.  There are other tools on GEDmatch for example, where you can compare DNA along with .gedcom genealogical files with other users - and look for shared segments on the chromosomes.  You can also compare your DNA to that of ancient populations.
  • Treat au-DNA differently to haplogroup results.  au-DNA is very interesting, and represents so much of our ancestry, if we could just sort some of the mess out.  You can partially do this by phasing your results with those of close relatives.  It is worthwhile phasing with at least one biological parent, if you can.  However, haplogroup results, provide by their mutations incredible stories over much longer periods - thousands of years.  A different kind of genealogy.  As we gather more data, and reference it also to ancient-DNA, so it will tell us more and more about two lines of descent.  Perhaps even into historical times.

The Thackers of Norfolk

The above photograph is of my Great Great Grandmother Sarah Anne Elizabeth Thacker (nee Daynes), of Rackheath, Norfolk.

I'm writing this for someone with shared chromosome segments that I've met on Gedmatch - my first potential relative, found through DNA comparisons.  She's in the USA, and has Thackers - which may be a link.  It's not that a common surname here, and very East English.  It has been suggested that the name is an Anglo-Danish variation of Thatcher.  I'm not so sure that it is of any Norse origin.  However, the strongest concentration of the surname in Britain today, is in the Dane Law county of Lincolnshire.  My Thackers though belong to a secondary cluster - also in the Dane Law, the region of East Anglia.

Thacker Family

I'm going to propose an estimated birth year of circa 1762 for my G.G.G.G.G grandfather John Thacker.  He first appears on my genealogical record on 2nd February 1787, when he married Mary Clarke at Rackheath in Norfolk.  However, tragedy struck.  Mary died a few years later in 1740, and was buried in the neighbouring parish of Salhouse.

John wasn't a widow for long.  He married my ancestor Ann Hewitt on the 26th April 1791 at Salhouse.

I have found records for three of their children.  Thomas Thacker was born in 1793, my G.G.G.G grandfather William Thacker was born 1796 at Salhouse, and his little sister Mary Thacker was born in 1801.

William Thacker married my ancestor Catharine Hagon at Rackheath on 19th May 1819.  Catharine gave birth to at least three children - William Thacker in 1820,my G.G.G grandmother Susan Thacker in 1823, and Thomas Thacker in 1825.  Their children were all later baptised at Salhouse Particular Baptist chapel.  I have very few Baptist ancstors.

William was an agricultural labourer, a farm worker by trade, as were the majority of my 18th and 19th century male ancestors.

Catharine must have died sometime between 1825 and 1833.

On 1st December 1833, William Thacker, now a widower, married Maria Cliborne at Rackheath.

Maria gave him at least three more children - George Thacker, born 1834, John Thacker, born 1837, and Ann Thacker, born 1841.

William Thacker died in 1874.

My G.G.G grandmother Susan Thacker, gave birth to my G.G grandfather, George Thacker in 1847 at Rackheath.  The father was not recorded.

George grew up to be an agricultural labourer and farm worker.  He married my ancestor Sarah Anne Elizabeth Daines (Photo above) in 1870.  Sarah (one of my mtDNA line) gave birth to at least ten children: George Thacker 1871, Ellen Thacker 1878, William Thacker 1876, my great grandmother Caroline Drusilla Thacker 1878, Catherine Thacker 1880, Thomas Thacker 1883, Rubin Thacker 1885, Walter Thacker 1887, Herbert Thacker 1891, and Rose Thacker 1893.

There is a family story about Sarah's strict paternal behaviour.  She was known as "Thacker by name, thacker by Nature" referring to "thacking" - to hit or punch hard.

My mt-DNA great grandmother Caroline married my great grandfather Samuel John Tammas-Tovell.

Exploring Gedmatch Eurogenes

The above grave is of my great great grandparents Robert and Ann Smith at Attleborough, Norfolk.

L1b Y-DNA News

First of all, it's looking good on the Y-Front.  My Y111 sample kit has arrived from FTDNA.  I also sent my 23andme V4 raw data to the administrator of the FTDNA Y Haplogroup.  He replied the next day "the raw data confirms that you are positive for M317 and negative for downstream SNPs M349 and M274. A very rare result for a NW European. It will be interesting to see who are your closest matches at 67 and 111 markers.".

So it doesn't look as though my L1b has anything to do with the M349 Rhine-Danube cluster.  I wonder where it comes from, how and when it got into an English ancestry?  It's starting to dawn on me just how rare it is in NW Europe.  European Y-Haplogroup maps and tables simply don't display or list it, because Y-DNA Hg L is not even considered a European Haplogroup, nevermind on British Haplo-maps.  All of those R1b's and I2's.  Not an L in sight.  I can see that having an unusual haplogroup is a mixed blessing.  Sure it's interesting, but no one knows much about it, because there is so little data on it in Europe, and so little research.

I had my first case of disbelief of my L1b Y-DNA on an FTDNA surname project group.  I reported my Y haplogroup as reported by 23andme (using ISOGG 2009) as L2*  The administrator retorted "It is NOT the "L" haplogroup, instead, it is "I".  So I linked her  copy of my 23andMe Paternal line report.  This time she replied  "Goodness gracious Paul. I administer many, many projects and yours is the first "L" You see, it has problems.

Wouldn't it be just great if I found someone else descended from the Berkshire Brookers by their Y line, that had the same haplogroup?

Gedmatch Eurogene admixture results for an Englishman

GEDMATCH offers free tools for analysing the autosome DNA of your raw data, from 23andme or Ancestry.com.  One suite of tools that are useful for analysing population admixture, are the Eurogene.  As an English person, with strong paper English ancestry - including almost certainly early medieval admixture, I thought that I'd get a comparison out of the way.  See which "works" best for my known ancestry and likely heritage.  I'm trying oracles on my 23andMe V4 raw data, for 1. EU Test, 2. K13,and 3. V2 K15.
1. Eurogenes EU Test
Oracle

1 Cornish 4.6
2 English 5.01
3 NL 6.26
4 West_&_Central_German  6.92
5 Orcadian 7.02
6 IE 7.33
7 FR 7.51
8 Scottish 7.95
9 DK 9.39
10 NO 11.57

A bit strange that it sees me as first "Cornish".  I don't know where it got that reference from.  I have no known Cornish ancestry.  However, 2 and 3 are likely.  As a whole it's not a bad prediction, just that the ball landed a bit to the West.
What about mixed populations?  What are it's favourite admixtures between two populations for me?

1   83.7% English  +  16.3% French_Basque  @  3.11
2   79% English  +  21% ES  @  3.17
3   63.7% English  +  36.3% FR  @  3.18
4   80.2% English  +  19.8% PT  @  3.5
5   51.8% FR  +  48.2% Scottish  @  3.54

Okay, not bad - it's given up on the Cornish.  However, it seems to point to France, Spain, and Portugal as a secondary source.  That is eerie, because 23andme threw up a speculative 2.4% South European including 0.5% Iberian.  I do wonder if I actually do have some unrecorded South European ancestry, even Iberian.

2. Eurogenes K13
Oracle

1 South_Dutch 3.89
2 Southeast_English 4.35
3 West_German 5.22
4 Southwest_English 6.24
5 Orcadian 6.97
6 French 7.63
7 North_Dutch 7.76
8 Danish 7.95
9 North_German 8.17
10 Irish 8.22

I like K13.  The Dutch may be there in admixture, and I know that they do often share some common patterns with SE English.  So I can excuse it making it to position 1.  Then in second place, the ball scores a goal.  Yes, I am SE English.  Most of the other suggestions could represent ancient admixture.

How about two population proposals?

1   65.6% Southeast_English  +  34.4% French  @  2.03
2   84.9% Southeast_English  +  15.1% North_Italian  @  2.05
3   63.5% Norwegian  +  36.5% Spanish_Valencia  @  2.06
4   69.7% North_Dutch  +  30.3% Spanish_Valencia  @  2.08
5   87.5% Southeast_English  +  12.5% Tuscan  @  2.09

It's got the SE English spot on, but all of these Iberians again!  Is it trying to tell me something?

3. Eurogenes EU Test V2 K15
Oracle

1 Southwest_English 2.7
2 South_Dutch 3.98
3 Southeast_English 4.33
4 Irish 6.23
5 West_German 6.25
6 North_Dutch 6.79
7 West_Scottish 6.84
8 French 6.85
9 North_German 6.89
10 Danish 7.26

Very good, except again, a bit skewed to SW England.  However, to be fair, I do have some slightly westward ancestors in the Oxfordshire area.  The rest is spot on.
What does it offer as a hybrid?

1   73.9% Southwest_English  +  26.1% French  @  1.27
2   71.8% North_Dutch  +  28.2% Spanish_Cantabria  @  1.3
3   89.7% Southwest_English  +  10.3% North_Italian  @  1.35
4   91.6% Southwest_English  +  8.4% Tuscan  @  1.4
5   86.4% Southwest_English  +  13.6% Spanish_Galicia  @  1.43

Those Spanish again!  Goes for SW English over SE English as the primary ancestral population.

Out of these predictions, my gut feeling is that they are all good for single population match.  On two population mix, they all suggest Iberian minorities.  Either I have an undiscovered South European ancestor, or something else is going on.  Do other English get this?  I can't really pick a winner.


Our exotic Y Haplogroup L1b

A Y haplogroup is a genetic marker that is passed down on a paternal line.  From great grandfather, to grandfather, to father, to son, and so on it goes.  The mt-DNA haplogroup on the other hand, is a genetic marker on the maternal line.  Together, they represent only two lines of descent.  The below illustration demonstrates these two markers on our own family pedigree fan chart over recent generations:


What is exciting about these two human haplogroups, is that by recording their mutations, and plotting them both against both the geographical distributions of present-day populations, and of archaeological human remains, we can start to paint a picture of past movements and origins in population genetics across thousands of years.  We can start to see how some of our ancestors moved across the World during prehistory.  Haplogroups offer a personal touch.

My recent 23andme test reported that I have inherited an mt-DNA haplogroup H6a1 from my mother, and a Y haplogroup L2* from my father.  

My brother, sisters, and my sister's children should also share the mt-DNA haplogroup H6a1.  This mt-DNA haplogroup has recently been recognised as originating in Eurasia.  It mutated from earlier haplogroups from Central Asia.  Current thought based on recent evidence (2015) suggests that it was carried into Western Europe during the early Bronze Age, circa 5,000 to 3,500 years ago, by pastoralists that spread out of the Eurasian Steppes north of the Black Sea in the Ukraine and South Russia area.  These Steppe pastoralists have been associated with an archaeological culture known as Yamnaya, and H6a1 has been detected in female human remains there.  Archaeologists suggest that their success was in domesticating strains of horses, that they could ride, in order to manage larger herds and flocks of grazing livestock.  Another success may have been their development of wheeled carts, that could be horse drawn.  Whatever the factors were, they appear to have been so successful, that their descendants spilled out from the Steppes, dominating Bronze Age Europe.  Therefore based on current evidence and thought, it might seem fair to imagine that we have direct maternal ancestors that 5,500 years ago were women in this Eurasian Steppe Culture.  That is the personal touch of the haplogroup.

But what about the Y haplogroup L2* that we inherited from our father, and our paternal line?  My brother and my son should share this Y haplogroup.  I'm making this post to better understand this heritage.

Y Haplogroup L


Distribution Haplogroup L Y-DNA

Distribution of Y haplogroup L today.  Above image by Crates (Own work) [GFDL (http://www.gnu.org/copyleft/fdl.html) or CC BY-SA 4.0-3.0-2.5-2.0-1.0 (http://creativecommons.org/licenses/by-sa/4.0-3.0-2.5-2.0-1.0)], via Wikimedia Commons.

There were a number of surprises from my personal 23andMe DNA test results.  However, that my Y haplogroup is L2* was perhaps the biggest shock.  I take back all reservations that I had about DNA testing for ancestral purposes.

The 23andMe introduction that accompanied my reported Y haplogroup suggested "Haplogroup L is found primarily in India, Pakistan and the Middle East. The L1 branch is especially common in India, while L2 and L3 are more common further north.".  This is not an English haplogroup.  It is not even a European haplogroup.  It is regarded here as South Asian, spreading down from Afghanistan to Sri Lanka, and across from Iran and into Eastern Turkey.  The above map illustrates the distribution of the Y Haplogroup L as we presently know it.  However, the Y haplogroup L has sub groups, that until recently were designated as L1, L2, and L3.  These subgroups were not distributed equally across the above geographic distribution.

"M76 (current L1a1, former L1) is the most common subgroup in India, while M76 and M357 (current L1a2, former L3) have approximately equal weight in Pakistan. M317 (current L1b, former L2) is rare in the Indian subcontinent. Iran seems to have all three major subgroups, while Turkey appears primarily M357. Other papers have found additional markers. For instance, L1b can be divided into two subgroups, M247 and M349. The people who do not belong to L1 have not been studied in academic papers, but only in personal genetic tests. Their ancestry is European, but it is possible that this group is present in the Middle East or Caucasus, where few people have tested". (Marco Cagetti).

My actual 23andme (ISOGG 2009) assigned L2* mutation should, using the latest designations, be referred to as L1b or, L-M317. I am seeing suggestions that L-M317 may have originated as recently as 10,000 years ago, between Levant and the Iranian plain. My haplogroup L-M317 appears to be strongest in clusters across Western Asia, between Iran and Turkey, with reports in Iraq, Armenia, Georgia, Anatolia, the Chechen Republic, and the Russian Federation.  It is not South Asian.  Marco Cagetti suggests that it is at very low frequency in Southern Europe, less than 1%.  However, this table might suggest that there are stronger pockets of Y Haplogroup L in pockets across Italy.  It has been observed in Portugal, Spain, Italy, and along the Mediterranean.  A sub-clade, L-M317 M349, is found in the Levant, but also clusters in in Central Europe including Germany, Austria, the Czech Republic, and Switzerland.  M349 is subsequently believed to have originated in the Levant.

What about in England?  L1b doesn't appear to have been well documented or researched here.  The FTDNA Y Haplogroup L Project has mapped only three L submissions in the UK - including one undisclosed, one M349, and a single L-M317 - this one in the Basingstoke area, not a hundred miles from my surname carriers in South Oxfordshire.

The chances are, that my L1b will pan out to belong to the L1b1a M349 sub-clade.  It could relate the Rhine-Danube cluster recorded in Central Europe at the FTDNA Y Haplogroup L project.

So how did it get here?  Where do the European L1b's come from?   Some researchers suggest that it could actually be in quite old in Europe.  It could have spread westwards out of the Levant with the Neolithic Revolution, carried by the first farmers.  If this is the case, then it may have been severely displaced by the arrival of new waves of haplogroups that arrived in Europe later, during the Early Bronze Age, leaving just a few clusters to survive.  My Y could be a remnant of earlier European farmers, that were largely displaced by the same wave of haplogroups from the Steppes that carried my mt-DNA into Europe.

Alternatively, it may have arrived here any time later - during the Later Neolithic, or as is a popular theory, it could have been spread into Europe from the Pontic Greek clusters around the Black Sea, or from elsewhere, via the Roman Empire.  It may have even spread into Europe during the medieval.  Some people suggest Byzantine movements in Southern Europe as one possible source.  Others claim links between their L1b and Ashkenazi Jews in their ancestry - either known, or suggested by autosome ancestry composition testing.

It has been suggested that I commission a BIG Y test, but I cannot justify that cost. I think that it is worthwhile commissioning a Y STR test, in order to examine and provenance it. Then should future research bring up any new understanding, I'll be able to best place our lineage within it.  I've ordered the FTDNA Y111 test next.

The 23andMe DNA results are in!

The results were uploaded to my 23andMe profile today.  I posted/registered the sample from the UK, nine weeks ago.  The sample traveled to the USA lab via a NL holding depot.  It took six weeks to process the sample and results, from the time of being marked as arriving at the USA lab.  I feel very fortunate, as many 23andMe customers are reporting a seasonal log-jam that is delaying the process.  My results though were comfortably within the proposed time frame.

There were a number of pleasant surprises.  The results were far from boring.  

Genetic Risk Factors

On the health side that we UK customers can presently still enjoy - there was only good news.  Although I have a family history of Alzheimer's that is strong on my father's side, there was no identification of any genes in my DNA, that have so far been associated with increased risk of the illness.  If my father did have these genes, I didn't receive them.  It does not mean that I will never be at risk to the illness, but it gives me some comfort.  Indeed, all of my 23andMe genetic risk factors were good.  There was no bad news.

Traits

An amusing little trait, that IS identified by the DNA analysis, is on Asparagus Metabolite Detection.  When I eat asparagus, my urine smells strongly.  It confirms for me - that the system works!  It also correctly identifies that I have a sweet tooth, that I have blue eyes, etc.

Now to the genetic genealogy goodies.

Ancestry

Y-DNA

The genetic marker that I inherit from my strictly paternal lineage - father's father, father, and so on, going back.  On paper, I've traced this back to a John Brooker, that lived in Oxfordshire, but was born outside of that county, perhaps in nearby Berkshire, circa 1785.  Of course, that is if no-one ever lied in forms over who the father was.

This one was a shocker.  A little background first.  Although my paper ancestry over the past 350 years is overwhelmingly localised in parts of the county of Norfolk, in East Anglia, my paternal-line surname carrier, that should be the donor of my Y chromosome marker, or Y-DNA, can be traced to Oxfordshire, in Wessex.  Out of my eight paper great grandparents, seven were Norfolk born and bred.  However, the exception was my paternal great grandfather.  Therefore I would not expect my Y-DNA to belong to any local Norfolk gene-pool.  It is the least representative lineage for my heritage.  This is why I feel that people can sometimes place too much value on their haplogroups.  I did however, expect it to belong to a common English or British haplogroup such as the Y-DNA R1b group.

I was in for a surprise.  It is exotic L2*.

From initial research including an Internet search, this haplogroup forms only a rare back scatter across Europe.  It appears more commonly across Western Asia and the Sub-Continent, from Turkey to Southern India.  It is most common in Pakistan, where it may originate, circa 30,000 years ago.  It is not a common European Y-DNA haplogroup.  I need to more carefully research this in the near future, but I'm in awe to find that I have an exotic Y-DNA.  It does conjure up images of one of my paternal ancestors being a Syrian archer, or Persian mercenary in the Roman Army, fathering a child, while stationed in Britannia, or perhaps elsewhere in Roman Europe.  But that might be too fanciful.  Anyway, I'm having pheasant curry for dinner tonight.

This genetic marker should be shared with my son, and my brother.  A few of my first cousins will also have it.

mt-DNA

The genetic marker that I inherit from my strictly maternal lineage - mother's mother, mother and so on back.  On paper, I've traced it back to a Mary Page, who was born in 1802, in Norfolk.  I like the maternal line, as it is actually the most biologically secure.  Few forms lie about who the mother is.  I'd expect my mt-DNA to be a haplogroup firmly established in East Anglia.

A nice one to have.  It is H6a1.

This haplogroup belongs to the Helena group.  However, it is not ancient European.  H6 is believed to have mutated from H around 30,000 years ago in Central Asia.

H6a1 has recently been associated with the Yamnaya migration into Western Europe, from the Eurasian Steppes to the north of the Black Sea, some 4,000 to 5,500 years ago.  In Europe itself, it could be associated with a number of Early Bronze Age cultures, the Corded Ware culture.  It has been linked with the R1b Y haplogroup, that dominates Western European countries such as Ireland, France, and the British Isles.  Recent studies have indeed suggested a significant displacement of people in Western Europe, that occurred in late prehistory, with the arrival of pastoralists from Eurasia.  This migration is also associated with the rise of the dominant Indo-European linguistic group of Europe.  If H6a1 does indeed prove to be linked to the Indo-European explosion of the early Bronze Age, I'd be very happy.  I like to imagine one of my maternal ancestors 5,500 years ago, accompanying a band of prehistoric pastoralists, that are heading westwards into Europe with their horses.

This genetic marker will be shared with my mother, my brother, my sisters, and their children.  A few cousins will also share it.

Ancestral Composition

This is an area that I've been trying to understand recently.  It uses computer analysis, to compare my autosome DNA to a number of others in reference populations from around the World, which then composes suggested ancestry in percentages.  This magic attempts to look not at a few genetic markers or haplogroups, but at all of the patterns in my autosomal DNA, to predict likely ancestry on any lineages that survive in my DNA.

Previous to receiving my results, I recently revised and bolstered up my paper genealogy based family tree,  I now have 172 direct ancestors listed, going back to Generation 14 during the 17th Century.  I noted that all, and everyone of my paper recorded ancestors were English.  All of them.  That includes all of my eight grandparents, all of my sixteen great great grandparents, and thirty of my thirty two great great great grandparents.  That is 100% English.

Now, I'm sure that you'd agree, I should be expecting my 23andMe ancestry composition to give 100% English, right? Well no.  They can't presently identify an ethnic group like the English.  Instead, I should expect my results to fall 100% into the British & Irish category.

100% British & Irish?  No, I'll give this one early.  it was 32% British & Irish on speculative mode.  More on this further down.

My paper research before I received my results also revealed just how concentrated, most of my ancestry has been over the past 350 years.  I compiled the below map of East Anglia.   The BLUE marking the places of ancestral events from my family tree on my father's side; and the RED marking the places of ancestral events on my mother's side.  The larger the marker, the more events recorded.

I also made a map based on East Norfolk during the 4th Century AD, before sea levels fell, and drainage changed the coastline.  I then marked out the area of my mother's ancestry on that.

 The point that I was trying to make was that I believe that my ancestry may have been more exposed to the North Sea Immigration waves of the 4th to 11th centuries AD.  More exposed than your average person of British & Irish heritage.  I also suggested that East Anglia, very much a part of the North Sea World, was particularly attractive to Early Medieval migrants from Frisia, Schleswig-Holsten / Angeln, North Saxony, and from Denmark.

On reviewing the 23andMe DNA Ancestry Composition of an admittedly small sample of other users with strong English heritage, I concluded that the average ethnic English person receives the results:

100% European

60% British & Irish

10% French & German

2% Scandinavian

25% unidentified broadly NW European

People of Irish heritage, or even Americans with either Irish or British ancestry, tend to score a higher percentage of British & Irish than do the present day ethnic English.  23andMe has a generous and growing reference population in it's British & Irish database.  However I hypothesised that 1) the 23andMe B&I reference is skewed to the Irish, and away from English.  It is also possible that it is distorted by a case of genetic drift by testing Americans of British origin.  2) that the British & Irish designation may actually be inadvertently looking at DNA that arrived in the British Isles largely previous to the early medieval North Sea migrations.  To the British and Irish genes that have been here since late prehistory.  On the other hand, the French & German, the Scandinavian, and perhaps some of the undesignated Broadly NW European percentages that are usually assigned to the ethnic English, may actually reflect early medieval migration from across the North Sea.  The computer analysis is simply unable to distinguish some of the DNA from that of present day French, Germans, or Scandinavians, because of ancient admixture.

I'm told that this would not be the case, that 23andMe ancestral composition could not detect such deep, ancient admixture.  However, what if I am correct about my own heritage - that I likely have enhanced levels of Anglo-Saxon and perhaps Norse heritage, because of the geographical location of so many of my ancestors?  Should I not expect even lower percentage of the 23andMe British & Irish category, and even higher percentages of other NW Europeans from across the North Sea?  So what was my 23andMe ancestry composition percentages (speculative mode)?

100% European.  Broken down into:

94% NW European.

3% South European.

I'll get to the South European later, but what about this North west European?  Let's break it down into 23andMe's sub categories:

32% British & Irish

27% French & German

7% Scandinavian

29% undistinguished broadly NW European

Oh my goodness.  It correctly fits my prediction.  I have more than double the average percentage of F&G and Scand for English people.  Despite having a paper researched genealogy that is 100% English, 23andMe's ancestry composition based on a generous reference sample size of 1251 sets, gives me 32% British & Irish.

So a predicted, but still incredibly exciting result.  I'm chuffed to bits.  It does in my eyes, blow 23andMe's British & Irish designation out of the water though.  Their reference samples do not appear to match the East English.  Instead, their software misreads some of the English DNA for French & German, or Scandinavian.  I'm suggesting that this is because of ancient admixture, during the 4th to 11th centuries AD, with North Sea immigration.  I invite others to knock my suggestion down.

One more surprise from my Ancestry Composition:  A South European 2.7%.  Broken down into 23andMe's sub categories:

0.5% Iberian

2.4% undistinguished broadly South European

This looks real.  It appears that I have a small percentage of South European heritage.  Most likely from Spain, Portugal, or Basque.  I probably have Iberian ancestry that I have not yet detected using paper genealogy.  Either that, or it's an anomaly, a incorrect interpretation.

Neanderthal Ancestry

Finally, how much Neanderthal DNA do I have?  How much of my DNA was shared by the archaic humans that lived across parts of Eurasia, between 350,000 to 30,000 years ago?  Evidence of early admixture events between Neanderthal and anatomically modern human populations?

An estimated 2.9%.

That's just slightly above the average of 2.7% for modern Europeans.  So I am not more Neanderthal than most others.  Sorry to disappoint.

All in all, very happy that I spent the money.

East Anglian Ancestry

http://www.openstreetmap.org/copyright

The above map has been modified from an original copied from © OpenStreetMap contributors

I have plotted my ancestral places that are tagged in my Gramps genealogical GEDCOM database.  These Places represent events -births, baptisms, marriages, or residence, etc.  These Places  belong to my direct ancestors, although they may also include siblings of ancestors.  Overall, I have modified this map in order to illustrate the distribution of my East Anglian (almost entirely of the County of Norfolk, with a few over the border in Suffolk) ancestors over the past 350 years - as so far revealed by paper genealogical research.

The BLUE markers represent the places of my father's recorded ancestors.  The RED markers represent the places of my mother's recorded ancestors.  The more events recorded for any place, the larger the marker.  You can click on the image in order to see a full resolution image.

The RED markers include pretty much all events for my mother's ancestors, as presently recorded in my family history database.  She has no recorded ancestry from outside of Norfolk, for the past 350 years.  She has an incredibly strong Norfolk ancestry.  Particularly in the East of Norfolk.

The BLUE markers do not cover all of my father's recorded ancestors, as I have also detected ancestry for him in Oxfordshire, London, and possibly Lincolnshire.  These ancestors lived outside of the mapped area.

When my mother and father initially met each other in 1956, they believed that they came from quite different parts of Norfolk, from opposite sides of the City of Norwich, with my father moving from East Dereham to my mother's neighbourhood in the Hassingham area.  Yet this map suggests that over the past 350 years, some of their ancestors have lived much closer.  The chances of them both sharing common ancestry during the Medieval, or even more recently are good.

This might support the findings of the POBI (People of the British Isles) 2015 study, that not only emphasised the homogeneous nature of the English, but also suggested that the old Anglo-Saxon kingdoms could still be detected as localised gene pools to today.

I have previously created the below map, using red dappling to mark out the main zone of my mother's ancestry, onto a map of East Norfolk, as it would have appeared during the 5th Century AD, before sea levels fell, and drainage works created the more recent Norfolk coast:

My hypothesis is that my mother's ancestors clustered in an area of East Anglia, that would most likely have experienced an influx of North Sea immigration between the 4th and 11th centuries AD from Frisia, and perhaps Angeln and Denmark.

I also modified the below map from Wikimedia Commons.  Attribution is: By Nilfanion [CC BY-SA 3.0 (http://creativecommons.org/licenses/by-sa/3.0) or GFDL (http://www.gnu.org/copyleft/fdl.html)], via Wikimedia Commons.  This map, as the legend states, illustrates the distribution of my recorded direct ancestors (bother on my father's and mother's sides are in RED) across the wider area of England at a single generation level, based between 1756 to 1810.  It suggests a combined ancestry, concentrated in Norfolk, East Anglia, but with a few lineages in Wessex / Mercia.



Where do we come from?

I can answer that now.  A set of maps that demonstrates the geographic spread of my direct ancestry back seven generations, to the early 18th Century.

I used a cropped relief map of England from Wikimedia Commons.  Attribution is: By Nilfanion [CC BY-SA 3.0 (http://creativecommons.org/licenses/by-sa/3.0) or GFDL (http://www.gnu.org/copyleft/fdl.html)], via Wikimedia Commons.

The red dots mark the locations of each ancestor, preferably a birth or baptism place, if not, then the next best provenance.

Grandparent Generation

All four ancestors of this generation are represented on this map.  All four are located in the county of Norfolk, in the East of England.  These ancestors were born between 1900 and 1910 in England only.  They represent two generations back from myself or my siblings.

Great Grandparent Generation

All eight ancestors of this generation are represented on this map.  Seven are located in Norfolk, in the East of England.  These ancestors were born between 1859 and 1885 in England only.  They represent three generations back from myself or my siblings.

Great Great Grandparent Generation

All sixteen ancestors of this generation are represented on this map.  They are concentrated in Norfolk again, but with single representatives each in Suffolk, Cambridgeshire, London, and Oxfordshire.  These ancestors were all born between 1830 and 1865 in England only.  They represent four generations back from myself or my siblings.


Great Great Great Grandparent Generation

Thirty of the thirty two ancestors of this generation are represented on this map.  The other two were undeclared fathers.  The main cluster is still in Norfolk, with a particularly dense cluster in the east of the county, around the River Yare.  Outside of East Anglia, I also had ancestors at this generation in Oxfordshire, London, and Lincolnshire.  These ancestors were all born between 1794 and 1837 in England.  They represent five generations back from myself or my siblings.

Great Great Great Great Grandparent Generation

Now the paper ancestry starts to fade away, with only 42 provenance ancestors out of 64 biological ancestors for this generation (seven generations back).  Therefore the map might lose some detail.  None-the-less, it seems to show the pattern settling, with most of my ancestry only deepening in Norfolk, and strongly clustering around the River Yare in East Norfolk.  Almost entirely restricted to East Anglia, except for a few emerging clusters in Wessex.

Surnames

The recorded surnames of my known direct ancestors are overwhelmingly of Medieval English form: 

Brooker, Curtis, Smith, Thacker, Tovell, Tammas, Hewitt, Lawn, Peach, Goffen, Norton, Barber, Baxter, Ellis, Hagon, Porter, Becket, Shawers, Key, Rose, Ford, Daynes, Quantrill, Crutchfield, Freeman, Larke, Waters, Ransby, Ling, Rose, Riches, Snelling, Merrison, Cossey, Shepherd, Durran, Edney, Hedges, Dove, Britiff, Harris, Tibnum, Mitchells.Briggs, Nicholes

The surnames Tovell, Thacker, Daynes, Ransby, and Hagon - all from my mother's Norfolk side, could hint at an Anglo-Danish influence.

Fan Chart up to most recent six generations:

Earlier Origins

The years and generations represented on the maps pretty much cover the past three hundred years of industrialisation and globalisation.  Much earlier, I'd expect less movement.  Therefore I feel that it would be safe to assume, that back to at least the medieval period, that my ancestry was concentrated in East Anglia, with a secondary patch in the Wessex area of England.  The recent POBA (People of the British Isles) 2015 study, suggested that the old Anglo-Saxon kingdoms continued to act as localised gene pools into the high medieval period.

Before that, we had a period of immigration waves into lowland Britain.  The POBI study, supported a number of other recent studies based on genetic profiling, archaeology, and place-name study, to suggest that Anglo-Saxon immigration accounted for no more than 30% to 40% of lowland British DNA, and that the majority of English heritage had existed in the British Isles previous - perhaps to influxes of genes during the Bronze Age or earlier.  Genetic profiling of human remains in Cambridgeshire, of people identified as 5th Century immigrant (Anglo-Saxon), suggests the closest present day profile as Dutch or / and Danish.  The kingdom of East Anglia identified with the Angles ethnicity, that historically provenance their origins to the region of Angeln, on the Danish and German borders on the Baltic coast.  However how elites identify their origin, is often not based in fact, neither is their origin always shared by their subjects.

East Anglia fell to the Danish army, and subsequently to Danelaw control periodically during the late 9th to early 11th centuries.  Some parts of East Norfolk such as Flegg, are particularly rich in Old Danish place-names.  POBA 2015 failed to identify a Danish presence with their genetic profiling, but the place-name evidence and historical sources contradict this finding.  The 7th to 9th centuries saw a slight reduction in sea levels, that enabled the draining of new lands in East Anglia for settlement.  The same districts are rich in Old Danish place-names, strongly suggesting immigrant settlement.

Conclusion

POBI 2015 suggests that I have ancestors that have lived in lowland Britain, since at least the Bronze Age, and most likely, much earlier.  That very likely ties me to lowland British ethnicities of the Bronze and Iron Ages.  The dominant power in East Anglia during the Later Iron Age was the Iceni federation, famous for the Boudiccan revolt against Rome.

POBI 2015 and other studies, suggests an Anglo-Saxon immigration that accounts for 30% - 40% of English ancestry.  My strongest cluster is concentrated in the river valleys of East Norfolk, exactly the sort of landscape that I would expect any North Sea immigrants during the 4th to 11th centuries to concentrate.  Therefore, I would expect a high probability of actual Anglo-Saxon immigrant ancestry (based on recent studies, from the Netherlands area, and perhaps North Germany / Denmark).  Based on place-name evidence the area was later heavily influenced by the Danish.

When I receive my 23andMe DNA results, based on their genetic profiling of Y chromosome, mtDNA, and on general autosomal calculators, in their ancestry results, I would expect to see overwhelming British & Irish percentage.  However, will their autosome crunchers also predict a percentage in the Scandinavian, French & German, and North-West European 23andMe categories?  As autosomal DNA is so random, what will the results display?

23andMe

Still waiting for the results.  23andMe are not giving a very rapid service.  For starters, I received a sample kit with a Netherlands return address.  That apparently was a holding depot, where they stockpile some of the European samples, so that they can ship them to the USA cheaper.  My sample reached a US lab, but continues to sit in a queue.  It has now been 37 days since I sent my registered sample off, and the box is still in a queue, waiting to be tested.  Other customers are reporting some long waits further down the process in quality control.  I expect a long wait.

Pardon my English

The 21st Century English are having an identity crisis.  No, I'm not likely to start raving some sort of nationalist or xenophobic agenda.  That isn't me.

Brexit may be taking us to the collapse not only of the European Union, but also to the collapse of the British Union.  I cant think of a time when it is more appropriate to consider, who are we?  Who are the English?

English identity has become entwined with the British Union for so long, and so deeply, that it has submerged.  Our nationality is British.  So what is English?  Living on a Thetford council estate several years ago during an International football tournament, I could see the red crossed flags all around me, with gangs of youths shouting out Inger-lund!  Yet the St George doesn't even have any official recognition within the United Kingdom.  When Scotland leaves the Union following Brexit, we might need to consider that.  Football fans seemed to connect with their version of Englishness increasingly from the nineteen nineties on.  In politics, this has transferred to a newly emerged English right wing "nationalism", as expressed by groups such as the English Defence League.  The term Little Englander can be employed to belittle such a stance.  English pride emerges decades after the collapse of the Old British Empire.  Like a cargo cult, perhaps driven on by some sort of fear.