DNA-4-Ancestry Test Comparison. Reviewing the DNA Companies. Updated to 2024


Comparing results from actual recorded ancestry, to that predicted by Ancestry.com, 23andme, My Heritage, Living DNA, FT-DNA and more.

Recorded Ancestry

I have researched my genealogy for circa forty years on and off. Since back in the day of interviews, visits to church yards, county archives, and London based archives. Before any internet genealogy, never mind genetic genealogy. Is it perfect? Of course not. I have a family tree of 6,000 family members. I've forgotten how many direct ancestors but certainly well over 300. Stretching back to the 16th century (1500s) in a number of places. The majority (thankfully) is very local to myself. Ancestry reports that I have based it on 19,600 records. I have certainly referenced my resources, and do not restrict them to the Internet. I still sometimes visit archives, etc.

Genetic Genealogy by DNA matches at Ancestry.co.uk, 23andme, Living DNA, FT-DNA and MyHeritage support the recorded tree very well over the past several generations on all sides. Plenty of support from centimorgans of shared DNA with other testers.

I base my Recorded Ancestry percentages on Generation 6. That is my great great great grandparents.

97% were English
Most East Anglian Norfolk, with some East Midlands and the Oxfordshire area.
3% Swiss.

No others. No Irish, Scottish, Scandinavians, Italians, Greeks, French, Turkmen, Balochi, etc.

So what did the DNA companies tell me?

Results

Ancestry.com/co.uk did very well only if you take the England & NW Europe category to be 'English'. They've been quite thoughtful in generating this category. Because the problem with the SE English is that we are too close to Dutch, Norman, and Danish to tell apart. This is because we have long, deep roots in those regions. The sub regions of their genetic communities is very good. But the community of E India might be down to having a tested family member with paternal roots in Sri Lanka? Maybe not.

My Heritage does very well. Although they have little bias towards Ashkenazi and SW Asia that just shows up. Still, not bad, they have improved.

Living DNA, an English business. Dear oh dear, what goes on there? They have even assigned my yDNA to the entirely wrong haplogroup!

FT-DNA, no better. Despite being the premier business for testing haplogroups, their autosomal test lets them down

23andme? Wtf. I know their problem. I'm convinced that their 'British & Irish' dataset is full of Irish or Irish American? It does not understand SE English DNA, and splits us with Continental datasets. As for their sub regions, I suspect based on dodgy DNA from health companies, collected from modern postcodes. That would explain that it shows where East Anglian ancestry has moved to over the past few centuries, not where it came from.

WeGene is convinced that I am French. Non.

They are all to various extents confused by medieval migration. Because with so much East Anglian ancestry, I have links across the North West European Continent from the earlier medieval. The East Anglians are probably very like Frisians etc. Our DNA is all so similar that these tests cannot tell us apart.

Well the tests were ALL very good for assigning my ancestry as a European. And pretty good at seeing it as primarily NW European. That's good. BUT, below that level, none of these tests could be described as accurate.

Fanboys of these tests beware.


The above is a fan chart of my direct ancestry, that I made in 2018. The coloured areas are supported by DNA matching (genetic genealogy) although I have had volumes of additional matches since then.

Neolithic & Bronze Age Calculator (K11): Geneplaza

This is the K11 admixture calculator with rarer alleles created by Dilawer Kahn, now available for a small fee as a test on Geneplaza.  I had previously commissioned Dilawer to run my 23andme DNA raw data through the calculator, but this is a nicer presentation.  The test seeks to estimate ancient ancestry admixture using his rarer alleles principle.

My results:

Western European Hunter Gatherers

"These were the indigenous populations of Europe that substantially contributed to the genetics of modern Europeans. It is believed that these hunter gatherers arrived in Europe around 45000 years ago from the Near East.".

My Western European Hunter-gatherer admix is 21.7%

Neolithic European

"This population introduced farming to Europe during the Neolithic, and were very likely descended from Neolithic farmers from the Near East. Their genetic signature is best preserved in modern Sardinians and other southern Europeans.".

My Neolithic European admix is 21.7%

Neolithic Anatolian

"These early farmers from Anatolia from about 8000 years ago were the ancestors of the Early European farmers that introduced farming to SE Europe, and replaced the hunter-gatherer cultures that lived there.".

My Neolithic Anatolian admix is 16.4%

Andronova-Srubnaya

"The Andronovo culture is a collection of similar local Bronze Age cultures that flourished around 3000-4000 years ago in western Siberia and the west Asiatic steppe. This culture overlapped with the Srubna culture in the Volga-Ural region of Russia.".

My Andronova-Srubnaya admix is 14.6%

Yamnaya-Poltavka

"The Yamna culture (also known as the Pit Grave culture), was an early Bronze Age culture from the Pontic Eurasian steppe from around 5000 years ago. The Yamna culture is identified with the late Proto-Indo-Europeans, and is the strongest candidate for the homeland of the Proto-Indo-European language.

My Yamnaya-Poltavka admix is 12.6%

Neolithic-Chalcolithic Iran

"Based on Neolithic and chalcolithic period samples recovered from Northwest Iran. The farmers from the Zagros mountain Iran region descended from one of multiple, genetically differentiated hunter-gatherer populations in southwestern Asia.  They are estimated to have separated from Early Neolithic farmers in Anatolia some 46,000 to 77,000 years ago, and show affinities to modern-day Kurd, Iranian, Pakistani and Afghan populations.  The Neolithic Iranian references used for this component, were recovered from the Kurdistan region of Iran, and appear to be around 9000 years old. The Chalcolithic Iranian references have been dated to around 5000 years old.".

My Neolithic-Chalcolithic Iran admix is 7.6%

Neolithic-Bronze Age Levant

"Based on neolithic and bronze-age period samples recovered from the Levant area in the Middle-East. The references for the bronze age Levant farmer (BA) samples were recovered from the Ain Ghazal, Jordan area and were dated to about 4300 years ago.  The first farmers of the southern Levant (Israel and Jordan) and Zagros Mountains (Iran) were strongly genetically differentiated, and each descended from local hunter-gatherers. By the time of the Bronze Age, these two populations and Anatolian-related farmers had mixed with each other and with the hunter- gatherers of Europe to drastically reduce genetic differentiation. The impact of the Near Eastern farmers extended beyond the Near East: farmers related to those of Anatolia spread westward into Europe; farmers related to those of the Levant spread southward into East Africa; farmers related to those from Iran spread northward into the Eurasian steppe; and people related to both the early farmers of Iran and to the pastoralists of he Eurasian steppe spread eastward into South Asia.".

My Neolithic-Bronze Age Levant admix is 4.4%

Eastern Non-African

"Eastern Non Africans (ENAs) are one of the earliest splits from humans that migrated out of Africa to the Near East around 100,000 years ago. It is believed that ENAs split from the population in the Near East around 50,000 years ago. Populations such as the Andamanese Onge and Papuans are modern descendants of ENAs. The ENA component here is based on Papuan references.".

My Eastern Non-African admix is 1%

Discussion

Any autosomal DNA tests for ancestry admixture have to be understood under a number of conditions:
  1. Timeline.  for what period in the past is this ancestry being weighed?
  2. Population.  How are the proposed admixture populations quantified and distinguished?  Where did they live?  Are they associated with any archaeological culture?  What are the references - are they based on ancient DNA or inferred in modern populations?  What previous populations were they admixed from?

Admixture is repetitive.  What we are looking at are ancient migrations, population expansions (sometimes marked with culture, sometimes not), admixture - sometimes with strong sex bias, displacements - all in prehistory, long before any written record.  It's like rows of jars of mixed sweets, resulting by mixing, and remixing.  Even siblings can have slightly different "flavours".

How does the above K11 compare with some of my previous ancient admixture calculations?  How about the K7 Basal-rich for example?  It looks at a different admix, from an earlier time, but do they make sense together?

David Wesolowski's K7 Basal-rich test

Villabruna-related

The Villabruna cluster represents the DNA found in 13 individuals in Europe from after 14,000 years ago.  They were Late Ice Age hunter-gatherers.  They appear to have links with the Near East.  The current thought is that they replaced earlier groups of hunter-gatherers in Europe.  The DNA of people in the Middle East and Europe pulled together at this time, and they may represent an expansion from the South-East.  Much of the Aegean Sea would have been dry, with low sea levels (glaciation), so the migration may have been easy.  It is believed that they had dark skin, and blue eyes.  They were possibly, the last hunter-gatherers of Europe and the Middle East.  They may have contributed to our DNA both through or either, later Asian or European admixtures.

David gives the English average as 56.7%.  My result is 57.1%

Basal-rich

The Basal Eurasians are a hypothetical "ghost" population derived from DNA studies.  It is suggested that they splintered from other modern humans 45,000 years ago, presumably outside of Africa, somewhere around the Middle East.  They significantly contributed DNA to the Early Neolithic Farmers of the Fertile Crescent and Anatolia, and consequently, on to all of us modern West Eurasians.  

 David gives the English average as 26.5%.  My result is 28.8%

Ancient North Eurasian

Another Ice Age hunter-gatherer "Ghost" population, but this one has been associated with human remains and an Upper Palaeolithic culture (Mal'ta-Buret') at Lake Baikal, Siberia.  We know that it significantly contributes to modern West Eurasians, through earlier admixture on the Eurasian Steppes.  Copper Age pastoralists then carried it westwards into Europe with their later expansion.

David gives the English average as 16.6%.  My result is 14.0%

Comparing  K7 Basal-rich to K11 Neolithic & Bronze Age

The K7 Villabruna should relate I feel, to the K11 Western European Hunter-Gatherer.  It's quite different.  The K7 gives me 57%.  The K11 gives me only 22%.  Refer back to the Discussion further up.  Different admixes, different times.  I was surprised at the high Villabruna percentages when I recieved the results.  Some of that Villabruna could have gone into other later admixes that are represented in the K11 populations.

The K7 gave me a higher-than-average (for a North west European) percentage of Basal-rich at 29%.  This is an earlier ghost population, that hasn't yet been securely associated with ancient DNA or an archaeological culture, but has been inferred as a component of Early Neolithic Farmers in the Middle East and Anatolia.  That could have gone into some of the later K11 Neolithic populations such as Neolithic European (22%), Neolithic Anatolian (16%), and Neolithic-Bronze Age Levant (4%).

The K7 gave me 14% ANE (Ancient North Eurasian), which is low for a North West European.  The fashionable thought is that ANE went into Yamna-Poltavka Steppe as a significant component, before being carried into North West Europe by Copper Age Steppe pastoralists.  K11 gave me 13% Yamna, and 15% Andronovo.

Comparing my K11 results wnot with the K7, but with other K11 testers online, my results are not that atypical for a North west European at all.  I still fit in pretty well with other people of North West European ancestry.

Living DNA - June 2017 updates

Living DNA produced their first update.  An update by a "DNA for Ancestry" business can sound like an admission of failure.  To some, it could sound like a recall due to product failure.  "Your previous ancestry was a mistake".  This only applies if you have bought into some marketing campaigns, that autosomal DNA tests for ancestry actually work even close to 100%.  Surprise, they don't!  They are cutting edge, in development, and far from accurate below a Continental level. They are still somewhere in the twilight between being nothing more than a genetic lottery, and actually becoming a tool that is useful.  Therefore "updates" are to be welcomed.  They are a sign that the business wants to improve the test accuracy.  That is to the credit of Living DNA.

My latest results?  First of all, a quick recap on my actual ancestry, as supported by family history, local history, ethnicity, and by a traditionally researched record based family tree that includes over 270 direct ancestors over the past 380 years.  I'm English.  Indeed, all of my direct ancestors, appear to have been South East English.  More precise, I'm East Anglian.  On family history and recorded genealogy, I'd suggest that between 75% and 85% of my direct ancestors over the past three centuries were East Anglian, almost all from the County of Norfolk.  Others on my father's side, if not in East Anglia, still in Southern England.

That I feel, makes me an interesting subject for ancestral auDNA testing.  You see, my ancestry is very localised here in South East England.  DNA tests such as 23andMe that claim to accurately plot ancestry over the past 300 - 500 years should get me.  But they don't.  This is because their algorythms, and reference data set designs fail over different ages.  They also (although they sometimes deny it), fail to discriminate against older population background.  We East Anglians and South East English have been heavily admixed with non-British populations on the European Continent.  Not so much over the past 500 years, so much as over the past few thousand years.

The new Results.

Below are my Living DNA regional ancestry, based on Standard Mode.

Below are my Standard Mode results broken down into sub regions.

Below is a table, comparing my recorded ancestry, with my early Living DNA results in Standard, now my revised results.

Living DNA has now introduced two new modes of confidence called complete and cautious modes.  First the Complete results:

Below are my Complete Mode results in regional:

Below are my Complete Mode results for sub-regional:

Now the Cautious results:

Below are my Cautious Mode results in regional:

Finally, below are my Cautious Mode results for sub-regional:

Conclusions

No auDNA test, by any DNA-for-ancestry company has yet come close to assigning me 100% English or even British.  They don't get me.  23andMe gives me 32-37% "British & Irish".  FT-DNA gives me "36% British".  Therefore, to be fair, Living DNA, giving me 70% "Great Britain or Ireland", give me the best result.  However, Living DNA has started out with the largest, best quality British data-set of any DNA-for-ancestry company, and is often accused of a bias towards Britain in it's results.  If so, then my 70% still looks weak.  They are planning on producing similar quality data sets soon for Ireland, Germany, then France. Therefore any results, will as I started out saying at the beginning of this post, be perpetually progressive.  Businesses that do not improve data sets or algorithms, will not get any better.  They are not progressive.

I get Southern European in other tests besides this one.  Living DNA points to Tuscany.  FT-DNA before a recent update gave me 32% Southern European, although they have revised this down to a little noise from South-East Europe!  23andMe gives me 2% Southern European - but this appears nothing unusual for an English tester.  None-the-less, I am interested in trying to better understand, why some of these tests give me this "Southern European" admixture, for which my family history, local history, and recorded genealogy has absolutely no account.  It equally reflects in ancient calculators that give me a little bit more Neolithic Farmer than for other English, which on average, already have a little more Neolithic Farmer than other British or Irish populations do.

The New Complete and Cautious Modes

How do I feel about these?  At Sub-Regional level, the Complete mode starts to get silly.  For the first time, Living DNA at this level, starts to even suggest some ancestry from Wales, SW Scotland, and Northern Ireland.  Only small percentages - but I just don't buy them.

However, the Cautious Mode, I start to like.  My British ancestry doesn't increase, but it looks more realistic, although with strange enigmatic suggestions still of Italian ancestry in the mix.  At Sub-Regional level, Cautious Mode also looks a little more likely.  My East Anglian remains at 37%, I however, lose Lincolnshire (which does exist in my record), but retain Cornwall.  I think Cornwall unlikely - however, there is just a small hint that something could be there, in surname evidence of a brick-walled great great great grandparent.  So maybe, just maybe.

East Anglia

I seriously doubt that my East Anglian ancestry over the past 300 years genuinely falls much below 75%.  Living DNA only appears to recognise a half of it at 37% - but they claim to be easily able to identify East Anglian DNA.  They call it "Distinct" because of it's high levels of Continental admixture.  They have admitted that based on their early data sets, that it was hard to separate from Germanic.  I don't know why it isn't stronger in my results.  I honestly do believe that the test underplays it on my results, even though it is the strongest of any population in my test results.  My East Anglian ancestors lived mainly in Eastern, Central, and Southern Norfolk.

Living DNA also provide a chart of the Continental "contributing regions" to East Anglian ancestry:

Finally, a chart breaking down their proposal of my British ancestry at Cautious mode:

I'm not disappointed with Living DNA.  That it does identify me as 37% East Anglian is I believe, incredibly good, and far advanced over any other DNA-for-ancestry test.  I'm looking forward to more updates in the future.  Well done Living DNA.

FT-DNA Family Finder My Origins 2.0 - April 2017 update

If there is anyone out there reading this blog, you know my recorded ancestry - all SE English, mainly East Anglian. No recorded evidence of anything but English over the past two or three centuries. This is not to say that I don't think any actually happened.




51% British might seem low for an Englishman - but I'm aware that my personal DNA flavour is a bit atypical for a Brit, more Continental. My Origins 1.0 gave me 36% British. 23andMe un-phased gives me 32% British / Irish. I do however suspect that my flavour isn't so atypical for an East Anglian of local rural ancestry. Living DNA gave me the most, a whopping 74% British. Therefore on that score, you could say that for myself, My Origins 2.0 actually comes in at 2nd place - better than 23andMe, DNA.land, or WeGene. I'm currently waiting for Ancestry.com results, but I'm not expecting better.

46% West and Central European where I have no record of any such ancestry - but East Anglian has been noted as close to North German, and certainly, SE England has plenty of early medieval admixture from that part of the world during the Anglo-Saxon event. In addition, we've continued to have immigration from the Continent over the past several hundred years, particularly but not exclusively, from the Netherlands and Northern France. I recently noticed that a 5xgreat grandparent had the surname Moll that is often found in Germany. However, it is also found in East Anglia, but are they connected? One day I'll find a recorded non-English ancestor! So as an East Anglian, I forgive autosomal DNA for ancestry algorythms that suggest that I have Dutch, German, French, or Danish ancestry. 23andMe (un-phased) gave me 27% French & German". Even Living DNA gave me 4.6% Scandinavian and 2% Germanic.

Now the Traces. I find these really interesting. Because they could fit in with other evidence. The My Origins 2.0 "Southeast European" designation appears to include Italy. My Origins 1.0 gave me a very silly 32% Southern European. 23andMe gave me 2% Southern European (although I have noted that the majority of English testers get a small percentage of this). Living DNA gave me a whopping 9.6% Tuscany. A friendly discussion with one of the LDNA techs, suggested that it looked to them, to be genuine. There was a family story on my father's side, that there was a "foreigner" - but I've never found any recorded evidence. I've scanned and scanned the tree for any sign, but nada. Not in great gp to 3 x great gp range. I'm open to a possible NPE, but I need more evidence than one auDNA test result.

The trace West Middle East and Ashkenazi are interesting, because although I have no recorded West Middle East or Ashkenazi ancestry, my Y-DNA does originate in SW Asia, possibly the area of Iran or Iraq. However, no auDNA test or GEDmatch calculator so far has provided any surviving evidence in the autosomes of any Asian, above that of average for a Brit. It all appeared washed out by genetic recombination. I share my Y with another family (different surname) from England, and we trace our lines back to the 1740's in Southern England (32 miles apart). That to me suggests that our immigrant Y ancestor most likely arrived in Southern England at least 400-500 years ago. I suspect earlier, maybe Medieval or even Roman. However, has the new algorithm picked something up? Maybe just a coincidence. The nearest non-English STR tester to us hailed from South Khorasan, Iran

A better prediction for myself than the My Origins 1.0 (below).

My Global 10 Genetic Map - and Frenchness!

My Global 10 Genetic Map coordinates:  PC1,PC2,PC3,PC4,PC5,PC6,PC7,PC8,PC9,PC10 ,0.019,0.0272,0.0002,-0.0275,-0.0055,0.0242,0.0241,-0.0033,-0.0029,0.0015

This is my position on the latest genetic map by David Wesolowski, of the Eurogenes Blog.  One point of interest that has been picked up on the Anthrogenica Forums, is my consistent closeness in ancestral results, to a Normand member!  Our Basal-rich K7 results were almost identical.  On 23andMe Ancestry Composition (spec mode), I just get a bit more French & German, while he gets just a bit more British & Irish.  We are close!

Another forum member argued though that it's my results that are skewed away from British, and towards North French.  He generated this map, plotting myself (marked as Norfolk in red), and my Norman Ancestral DNA twin Helge in yellow:

I had to point out though, that I've rarely seen other SE English with a record of local ancestry, test - and that the red circles representing British & Irish include many people with some Irish, Scottish, Welsh, Western, or Northern ancestry.  The map suggests a pull to Northern France, Belgium, and the Netherlands.

As I commented towards the end of my last post, I initially expected a pull to Denmark, Northern Germany, and perhaps to the Netherlands.  This is because so many of my 17th-20th century ancestors lived on what was the frontier of Anglo-Saxon and Danish immigration during the 4th to 11th centuries.

But instead, autosomal DNA tests for ancestry all seem to be suggesting more shared ancestry from a more southerly direction - Northern France and Belgium particularly.  Although there has so far been a dearth of local testers from local families, the POBI survey seems to find this common among the English.  We appear to be a halfway house between Old British, and the French, more than the ancestors of Anglo-Saxons and Danes.  This contradicts the historical and archaeological records.  POBI suggested that this was due to waves of unrecorded immigration from the South during late prehistory.  Others have pointed the finger at Norman and French admixture in medieval Southern Britain.  It could be both!

Can I apply for French citizenship?

Autosomal DNA Tests for Genealogy

First a disclaimer.  I'm very new to the whole world of genetic genealogy.  I'm not new however, to traditional genealogy, and I do have a pretty good amateur understanding of relative archaeological and anthropological discussions over the past fifty years.  The following is not meant as a critique of genetic genealogy, so much as a review, or my experience, of ancestry composition based on autosomal DNA analysis.

Let's start with my paper trail.

Traditional Genealogy

I am English by ethnicity, British by nationality, and a subject of Queen Elizabeth II (often now referred to as a UK Citizen).

My paper recorded ancestry consists of the genealogical records of:

  • Generation 1 has 1 individual. (100.00%)
  • Generation 2 has 2 individuals. (100.00%)
  • Generation 3 has 4 individuals. (100.00%)
  • Generation 4 has 8 individuals. (100.00%)
  • Generation 5 has 16 individuals. (100.00%)
  • Generation 6 has 29 individuals. (90.62%)
  • Generation 7 has 49 individuals. (76.56%)
  • Generation 8 has 35 individuals. (27.34%)
  • Generation 9 has 24 individuals. (10.16%)
  • Generation 10 has 10 individuals. (2.34%)
  • Generation 11 has 4 individuals. (0.39%)
  • Total ancestors in generations 2 to 11 is 181. (9.04%)

All 181 ancestors, reaching back to the 1690's, appear to be English born, of English ethnicity, with English surnames.  The majority of them (100% on my mother's side, and 81% on my father's side) were East Anglian, with the vast majority of that percentage being born in the county of Norfolk.  Religions recorded or indicated were CofE Anglican or non-conformist Christian.  No sign of any Catholicism, Islam, or Judaism.

Therefore it would look pretty likely, that I can claim English heritage, wouldn't you agree?

Genetic Genealogy and Ancestry Prediction

There are three aspects or avenues of inquiry, available for genetic genealogy.  First of all, the two sex haplogroups; the y-DNA, and the mt-DNA. These two "signals" are referred to as haplogroups.

  1. The y-DNA.  This follows the Y chromosome.  It is only carried by men.  It is passed along the paternal line, and only by that line, from grandfather, down to father, down to son, until the line is broken.  What a lot of people do often misunderstand, is that it does not represent 50% of your ancestry.  It does not represent all of your biological father's ancestry.  For example, his mother's father, and her brothers, although on your father's side, would most likely carry a different y-DNA haplogroup.  It only comes down an uninterrupted strictly paternal line.  Even at Generation 7 (g.g.g.g grandparents) above, it would have been carried by one out of my sixty four biological ancestors at that generation.  The other thirty one g.g.g.g grandfathers for that generation may have carried different Y haplogroups.
  2. The mt-DNA.  Although a very different type of DNA, this one works as the opposite sex haplogroup.  It is a signal that is passed down the strictly maternal line, from grandmother, to mother, to her children.  Yes, we men do inherit our mother's mt_DNA, but we can't pass it down.  Only our sisters can.
  3. The au-DNA, better known as Autosomal DNA.  Whereas the former two sex haplogroups are handy, because we can measure their mutations, and track their formation and movement across thousands of years, au-DNA really is the stuff that we are made of - all of the SNPs on our chromosomes that personalise us within the human genome.  We inherit our au-DNA from all of our recent ancestors.  Roughly 50% from our biological mother, and 50% from our biological father.  Equally, we could say on average, 25% from each grandparent, or 12.5% from each great grandparent.  However, it is messy.  At every reproduction (meiosis), it gets messed up by recombination.  Not only that, but go back much more than six generations, and it becomes more and more likely that you can lose entire lineages.  You can have no surviving trace of any DNA from for example, a particular g.g.g.g.g grandparent.

Autosomal DNA is what makes us individuals, gives us our hereditary traits.  It is passed down from many ancestors, via our parents.  However, the sex haplogroups are of interest because they can be traced across the globe, and the millennia.  As we gain more and more data - both from living populations, and ancient DNA from archaeological finds, so we will be able to track the STR and SNP mutation data more precisely.

However, what about poor old messed up autosomal DNA?  It represents our entire biological heritage over many generations. It is what we are. However, making sense of it is less easy, less precise.  Genetic genealogists are making progress, but it is far less of a precise science than either of the haplogroups.  They use calculators, that measure the segments of DNA cross the chromosomes, looking for patterns that they recognise from a number of known reference populations.  From that, these calculators predict an ancestry.  Exactly what and when that ancestry refers to, does seem to vary from one calculator to another.  There is an argument that the precision can be improved if you also test close known relatives including at least one parent.  The results can then be phased.  I'm actually waiting for the results for my mother, so that I can see my own au-DNA ancestry results phased and corrected.

So lets have a bit of fun, and see what some of the calculators suggest for my autosomal DNA, at least before any phasing with my mother's DNA.  What do they make of my 100% English paper ancestry?

23andMe.com Ancestry Composition Standard Mode

99.9% European.

Broken into:

83% NW European

17% Broadly (unassigned) European

I think that's pretty cool.  As I'm getting to know au-DNA predictions, so as I'm learning to appreciate it when they get the right continent, and the right corner of that continent.  That is more than they could do a decade or two ago.  The prediction is correct, I am a NW European.  I'm not a West African, a South Asian, or a East Siberian.

23andMe.com Ancestry Composition Speculative Mode

100% European

Broken into:

94% NW European

3% S European

3% Broadly (unassigned) European.

Whoa, where did that South European come from?  It could just be a stray incorrectly identified signal, or it could be telling me that one of my ancestors, maybe around Generation 6, were from down south!  Lets break down the prediction further.  First, the NW European:

32% British & Irish

27% French & German

7% Scandinavian

But surely I should be 100% British & Irish?  Not only 32%.  I have my own ideas about this.  I think that although 23andMe claims that Ancestry Composition only represents the ancestry of the past 300 to 500 years (the so-called migration period, as sold to USA customers), that it gets confused by earlier migrations across their reference populations, including those during the early medieval period, and perhaps even some of those during late prehistory.  I've noticed that across Ireland and Britain, the further to the east, the more diluted the 23andMe British & Irish assignment.  People of solid Irish ancestry get between 85% and 98% British & Irish.  My East Anglian results, mixed between British & Irish, French & German, and Scandinavian, are actually rather more like those received by Dutch customers of 23andMe.

As for that Southern European prediction, how does that break down?

0.5% Iberian

2.4% Broadly (unassigned) South European.

Which if taken seriously, might suggest that I have an unknown Spanish or Portuguese ancestor around Generation 6.  If I did take it seriously that is.  I wonder what my mother's test will reveal?

DNA.Land.com Ancestry Composition

This is a third party site, that you can upload your 23andMe V4 raw data to, and see what their calculators predict for your ancestry.  It has recently had it's ancestry composition revised.  What did that make of my 100% English au-DNA?

West Eurasian 100%.

I like that designation, the amateur anthropologist in me prefers that broad designation over "European".  Broken down:

77% North/Central European

19% South European

2.4% Finnish

1.3% unassigned.

What?  Why not 100% North/Central European?  Finnish?  Did some early medieval Scandinavian settlers of East Anglia bring it?  Or is it a false signal?  Misidentified au-DNA?

That darned South European kicked in again.  I'm here looking at a biological cuckoo NPE (non-parental event) at around Generation 5 or even more recent!  Did a great grandmother secretly have a South European lover?  But this South European breaks down further:

13% Balkan

6% Italian.

Oh my goodness, whereas 23andMe speculative mode suggested SW Europe - this one suggests SE Europe!  Do I have a secret Albanian great grandfather?  Or is it all nonsense?

WeGene.com

This is a cracking new third party DNA analyser.  It is based in China, and it's predictors appear to calculate mainly for a Chinese market.  It not only predicts your ancestry composition, but also your two sex haplogroups, and lots of traits and health predictions to compliment those of 23andMe.  It even tries to predict your genetic disposition to sexuality!

It will allow you to send your 23andMe V4 raw data direct to it's own calculators.  However, at the moment the website is almost entirely in Chinese (Mandarin?).  There are two options.  1) At the bottom of the webpages is a hyperlink to English, which gives, in English, a basic ancestry composition, and your haplogroups.  It does not include English versions of the health and trait results.  2) use an online translator, such as the one built into the Google Chrome browser.  It actually serves pretty well.

On sex haplogroups they give my Y-DNA as

L1.  Not bad, but they didn't make it to L1b or L-M317.

My mtDNA?

H6a1a8.  Very good.  Better than 23andMe's H6a1, and the same as the mthap program.

But this is about au-DNA, how did they do, what did they make of my 100% English ancestry?

81% French

19% English/Briton

Now, that sounds pretty awful, but on closer inspection, I'm impressed.  No South European great grandfather.  Okay, so most of my DNA has been placed on the wrong side of the Channel.  However, I know that French and English DNA is actually very close.  Recent surveys even suggest that the English have inherited a lot of common ancestry with the French during unknown migration late in prehistory.  So again - they very much got the right corner of the right Continent.  Well done WeGene.

GEDmatch.com Eurogenes K13

GEDmatch is a website that you can upload raw data not only from 23andMe, but from a range of testers, and from V3 chips as well as V4.  It hosts a number of tools and predictors - some Open Source.  Some of these predictors are for Admixture or ancestry composition.  They measure your ancestry in terms of distance from known reference populations.  The lower the number, the closer you are to their reference.  They use calculators known as oracles to predict ancestry, including mixed ancestry or admixture.

The oracles on the Eurogenes K13 and K15 calculator models have a good reputation at working with West Eurasian ancestry.  So how does K13 first, score my 100% English ancestry?

On Single Population Sharing, it rates my DNA against the closest references.  In order of closest to not so close, the top five are:

1 South_Dutch 3.89
2 Southeast_English 4.35
3 West_German 5.22
4 Southwest_English 6.24
5 Orcadian 6.97

I think that's a cracking result.  Okay, it thinks that I'm closer to South Dutch, than I am to SE English, but so close - and my East Anglian ancestry most likely does include a lot of admixture from the Low Countries from the early medieval period.  I really like Eurogenes K13.

Okay, let's now use the Oracle 4 option, to suggest admixture.  First on three populations admixing to create my DNA, what comes closest?

50% Southeast_English +25% Spanish_Valencia +25% Swedish @ 2.087456

Well that's interesting!  The SE English hit the net.  The Swedish?  Could be ancient Scandinavian admixture - but the Iberian prediction has reemerged!

On four populations admixing?

1 Southeast_English + Southeast_English + Spanish_Valencia + Swedish @ 2.087456
2 Southeast_English + Southeast_English + Spanish_Murcia + Swedish @ 2.147237
3 Norwegian + Portuguese + Southeast_English + Southeast_English @ 2.216714
4 Danish + Portuguese + Southeast_English + Southeast_English @ 2.225334
5 Portuguese + Southeast_English + Southeast_English + Swedish @ 2.230991

Oh my goodness.  K13 agrees with 23andMe AC, that I have an Iberian link.  I'm now really starting to wonder.

Let's finish off by trying K15 on my 100% English ancestry:

GEDmatch.com Eurogenes EU test V2 K15


Using Oracle for single population first, the top five closest:

1 Southwest_English 2.7
2 South_Dutch 3.98
3 Southeast_English 4.33
4 Irish 6.23
5 West_German 6.25

Okay, I'm SE English, not SW English, but pretty impressive again.

Using the oracle 4 for three population admixture, what mix comes closest to my auDNA?

50% Southwest_English +25% Spanish_Castilla_Y_Leon +25% West_Norwegian @ 1.080952

That Iberian back again!

Top five mix ups of populations closest to me?

1 Southwest_English + Southwest_English + Spanish_Castilla_Y_Leon + West_Norwegian @ 1.080952
2 Irish + North_Dutch + Southwest_English + Spanish_Galicia @ 1.111268
3 North_Dutch + Southwest_English + Spanish_Galicia + West_Scottish @ 1.282744
4 Southeast_English + Southwest_English + Spanish_Castilla_Y_Leon + West_Norwegian @ 1.295819
5 North_Dutch + North_Dutch + Southwest_English + Spanish_Castilla_Y_Leon @ 1.304939

I can't help preferring the K13 results to the EU test V2 K15 - simply because it recognises me better as SE English, rather than to their SW English reference.

Conclusions

If anyone ever bothers reading this far too lengthy post, I hope that I have imparted the following lessons:

  • Don't expect DNA Ancestry tests to pin point an actual country of ancestry.  They're not no where near that good yet.  The populations of West Eurasia, and elsewhere, are actually all mixed up, or share a lot of recent admixture.  In addition, many European nation-states are quite recent inventions.  I've seen the borders of Europe change in my short lifetime.
  • Don't expect precision.  If for example, you are an American, and a 23andMe AC test suggests only 32% British & Irish, then you could actually have 100% English ancestry over the past 300 years!  We're so mixed up, that these tests are struggling to part and identify us by nationality.
  • If you are willing to share your raw data (there are privacy issues), then have fun trying out all of these third party calculators.  It's a lot of fun as you can see.  They rarely agree.  There are other tools on GEDmatch for example, where you can compare DNA along with .gedcom genealogical files with other users - and look for shared segments on the chromosomes.  You can also compare your DNA to that of ancient populations.
  • Treat au-DNA differently to haplogroup results.  au-DNA is very interesting, and represents so much of our ancestry, if we could just sort some of the mess out.  You can partially do this by phasing your results with those of close relatives.  It is worthwhile phasing with at least one biological parent, if you can.  However, haplogroup results, provide by their mutations incredible stories over much longer periods - thousands of years.  A different kind of genealogy.  As we gather more data, and reference it also to ancient-DNA, so it will tell us more and more about two lines of descent.  Perhaps even into historical times.