DNA-4-Ancestry Test Comparison. Reviewing the DNA Companies. Updated to 2024


Comparing results from actual recorded ancestry, to that predicted by Ancestry.com, 23andme, My Heritage, Living DNA, FT-DNA and more.

Recorded Ancestry

I have researched my genealogy for circa forty years on and off. Since back in the day of interviews, visits to church yards, county archives, and London based archives. Before any internet genealogy, never mind genetic genealogy. Is it perfect? Of course not. I have a family tree of 6,000 family members. I've forgotten how many direct ancestors but certainly well over 300. Stretching back to the 16th century (1500s) in a number of places. The majority (thankfully) is very local to myself. Ancestry reports that I have based it on 19,600 records. I have certainly referenced my resources, and do not restrict them to the Internet. I still sometimes visit archives, etc.

Genetic Genealogy by DNA matches at Ancestry.co.uk, 23andme, Living DNA, FT-DNA and MyHeritage support the recorded tree very well over the past several generations on all sides. Plenty of support from centimorgans of shared DNA with other testers.

I base my Recorded Ancestry percentages on Generation 6. That is my great great great grandparents.

97% were English
Most East Anglian Norfolk, with some East Midlands and the Oxfordshire area.
3% Swiss.

No others. No Irish, Scottish, Scandinavians, Italians, Greeks, French, Turkmen, Balochi, etc.

So what did the DNA companies tell me?

Results

Ancestry.com/co.uk did very well only if you take the England & NW Europe category to be 'English'. They've been quite thoughtful in generating this category. Because the problem with the SE English is that we are too close to Dutch, Norman, and Danish to tell apart. This is because we have long, deep roots in those regions. The sub regions of their genetic communities is very good. But the community of E India might be down to having a tested family member with paternal roots in Sri Lanka? Maybe not.

My Heritage does very well. Although they have little bias towards Ashkenazi and SW Asia that just shows up. Still, not bad, they have improved.

Living DNA, an English business. Dear oh dear, what goes on there? They have even assigned my yDNA to the entirely wrong haplogroup!

FT-DNA, no better. Despite being the premier business for testing haplogroups, their autosomal test lets them down

23andme? Wtf. I know their problem. I'm convinced that their 'British & Irish' dataset is full of Irish or Irish American? It does not understand SE English DNA, and splits us with Continental datasets. As for their sub regions, I suspect based on dodgy DNA from health companies, collected from modern postcodes. That would explain that it shows where East Anglian ancestry has moved to over the past few centuries, not where it came from.

WeGene is convinced that I am French. Non.

They are all to various extents confused by medieval migration. Because with so much East Anglian ancestry, I have links across the North West European Continent from the earlier medieval. The East Anglians are probably very like Frisians etc. Our DNA is all so similar that these tests cannot tell us apart.

Well the tests were ALL very good for assigning my ancestry as a European. And pretty good at seeing it as primarily NW European. That's good. BUT, below that level, none of these tests could be described as accurate.

Fanboys of these tests beware.


The above is a fan chart of my direct ancestry, that I made in 2018. The coloured areas are supported by DNA matching (genetic genealogy) although I have had volumes of additional matches since then.

23andme 120 populations

Well I received my updated 23andme results

Here's the latest that 23andme gives me in their test. First my mother:

Her recorded ancestry is ALL East Anglian in SE England. 225 named in records, some lines going back to the 16th Century. Very localised, rural recorded and documented ancestry. No known ancestry other than British:







What she gives me with phasing:



My recorded ancestry by location:



At Generation 6: 97% SE English and 3% Swiss.

The rest of my 23andme report (V4 after phasing one parent):





A British grandparent? Absolutely, all four were!

A French or German great grandparent. I'm afraid not. At least this is an improvement on my old TimeLine that suggested a French or German grandparent, but still wrong.

Actually I had a Swiss 3rd great grandparent, but he was likely to have only given me 0% to 5% of my DNA.

A Scandinavian 4th or 5th great grandparent? Not impossible, but a little unlikely. Of course, most English get a little Scandinavian. Old admixture.

As for my mother's TimeLine. I know ONLY of East Anglian ancestors on record. Of course, she would have had some other ancestors at some point, but French / German, Scandinavian, in the past four or five generations? No. The African would be very cool. It's always possible - there were Africans around in very small numbers. But likely in rural Norfolk? Unfortunately not.

The new "dots.

I predicted Dutch for both of us. I thought I might also get Belgian or / and French. Not because I have recent ancestry from those places, but because they share much older common links with SE England. We are close.

No Irish - that's true, nor Scottish. So they did okay to eliminate that one. Finally, even though I get only 38% B&I (32% before phasing), 23andme awards me 4 out of 5 dots for Britain!

I guess that if I was to believe the line, then I had Dutch ancestors arrive here over the past 200 years. Perhaps Scandinavian a little further back, between 200 years and 500 years ago.

But I'm afraid I don't buy it.

Which is more accurate AncestryDNA or 23andMe?

I've been researching my ancestry, on and off, for about 30 years. That included interviewing elderly relatives, family histories, photographs, documents, etc. It progressed onto BMD certificates, Census, and many, many visits to local record offices, churches, and archives across Britain in order to examine parish registers, transcripts, minute books, etc.

These days I have the luxury of online genealogical resources, and the ability of searching online data bases. To cut a long story short, I have accumulated a family history that includes the names of 279 recorded, direct ancestors, 277, of which lived in South East England, particularly in the East Anglian County of Norfolk (the other two were a Swiss 3 x great grandparent and his named father).

At Generation 6 (3 x great grandparent), I can say that on paper, I am 97% South East English (including 75% East Anglian), and 3% Swiss. In other words, pretty much of local East Anglian ancestry. Here is a map showing my recorded ancestors - blue via my father (minus the few in Switzerland), red via my mother:

Okay, I will still have some mistakes in my genealogical research, particularly on more distant lines, where records start to be come more scarce and have less survival. There would also be some NPEs (non parental events). However, if I compare my pedigree with DNA matches / cousins that share common ancestors both in segments and on paper trails, I get this (shaded areas verified with DNA matches to paper trails):
So there is a reasonable verification there.

That is my background. The results? Remember, Generation 6:

97% English
3% Swiss

Well before phasing, 23andme gave me:

100% European: 94% NW European. 3% Southern European. 3% Broadly European. Broken down to:
32% British & Irish
27% French & German
7% Scandinavian
29% Broadly NW European
2% Broadly Southern European (including 0.5% Iberian)

After phasing with a surviving parent, it adjusted to:

100% European: 96% NW European. 2% Southern European. 2% Broadly European.
38% British & Irish (23% from father, 15% from mother)
24% French & German (13% from father, 11% from mother)
0.8% Scandinavian (from mother alone)
34% Broadly NW European (22% from father, 12% from mother)
2% Broadly Southern European (1% from father, 1% from mother)

Not very impressive is it?

AncestryDNA gave me:



Still way off, but a lot closer and more precise than 23andme. They also assigned me both to the Southern England Genetic Community, and to the East Anglia & Essex Genetic Community, perfectly correct.

Therefore in summary - I've come to the conclusion that NO current autosomal DNA test for ancestry is capable of accurately predicting your ancestry below a very large region, such as NW Europe - unless your ancestors belong to a particularly well defined population that avoided medieval admixture. They are all inaccurate. More important to me now is that they have fat databases of testers, with a system of searching them alongside family trees, ancestor locations and surnames. However, side by side - for my results, and weighed simply against recorded family history, I have to pronounce AncestryDNA/.com to be more accurate than 23andme.

Thoughts in understanding ancestry DNA

Above image.  My Global 10 Genetic Map coordinates:  PC1,PC2,PC3,PC4,PC5,PC6,PC7,PC8,PC9,PC10 ,0.019,0.0272,0.0002,-0.0275,-0.0055,0.0242,0.0241,-0.0033,-0.0029,0.0015.  The cross marks my position on a genetic map by David Wesolowski, of the Eurogenes Blog

The above map shows genetic distances between different human populations around the planet.  Look how tightly the Europeans cluster.  Razib Kahn recently blogged on just this subject.  The fact of the matter is that the greatest diversity exists between populations outside of Europe, particularly within Africa, and between African and non-African populations.  However, we obsess over tiny differences within European populations, when in truth, most Western Eurasians are very closely related.  We share ancient ancestry from slightly varied mixes of only three base ancestral groups, with the last layer arriving only 4,300 years ago.  This obsession in the Market drives DNA to the consumer businesses to largely ignore non-European diversity, and to focus too closely on differences that blur into each other.

The above image is from CARTA lecture. 2016. Johannes Krause of the Max Planck Institute. It shows the currently three known founder populations of Europeans and their average percentages.

However, at the same time the new Living DNA service seeks to zoom in closer on British populations, attempting to detect ancestry percentages from such tiny zones as "East Anglia".  They appear to be having a level of success with it as well, although that blurriness, that overlap and closeness of populations in Europe gives problems.  Germans are given false percentages of British, Some Scottish appear as Northern Irish, and the Irish dilute into false British areas.  However, I've seen enough results now to suggest that it is far from genetic astrology.  They get it correct to a certain level, particularly for us with English ancestry.  Ancestry DNA customers expect perfection.  I don't think that we will ever get that from such closely related populations at this resolution, but it does provide a new genealogical tool that can point us into some revealing directions.

Above image.  My Living DNA Map.  Based on my recorded genealogy, I estimate 77% to 85% East Anglian ancestry over the past 250 years or so.  Living DNA at Standard Mode gave me 39%.  I'm impressed by that.  That a DNA test can recognise even at a 50% success, my recent ancestry in such a tiny zone of the planet.  I have doubts though that this sort of test will ever be free of errors, and mistakes.  The safest DNA test for ancestry is still one that is based on more distinct populations, and outside of Africa, that can be as wide as "European".  23andMe for example in their "Standard Mode" (75% confidence), assign me 97.3% European, and 0.3% Unassigned.  That is a pretty safe result.

Autosomal DNA tests for ancestry, particularly for West Eurasian (European and Western Asia) descendants, are not reliable at high resolution.  If you want to get really local, then sure - do it.  However, only use the results as an indication, not as a truth.  Populations in Western Eurasia are closely related, and share recent common descent.  There has been a high degree of mobility and admixture ever since.  Some modern populations tested do not have a high level of deep rooted local ancestry in that region.  They overlap with each other.  Keep researching and meander through different perspectives of what your older pre-recorded ancestry could have been.

Above image by Anthrogenica board member Tolan.  Based on 23andMe AC results.  My results skew away from British, and towards North French.  He generated this map, plotting myself (marked as Norfolk in red), and my Normand Ancestral DNA twin Helge in yellow.  My results fall in the overlap with French.  Helge is Normand but in AC appears more British than myself.  I am East Anglian yet in this test appear more French than he does.



Will ancestry DNA tests tell me my family origins?

I have taken several DNA tests for ancestry, including those provided by the FT-DNA, 23andMe, and Living DNA companies.  Unusual for a tester, I am actually of a single population, very local, well documented ancestry here in East Anglia, South-East England.  I'm not someone in the Americas or Australia, that might have very little clue what parts of the world that their ancestors lived in, previous to immigration.  I know my roots, I'm lucky.  I live them.  You might ask, why did I feel the need to test DNA for ancestry?  The answer is, curiosity, to test the documented evidence, fill the gaps, look for surprises, and in particular, to understand the longer term, to reach further back into my ancestry.

I have though, become a bit of a skeptic, even a critic, of autosomal DNA (auDNA) tests for ancestry.  They are the tests presented by the businesses in results called something like Ancestry, Family Ancestry, Origins, Family, Composition, etc.  Instead of testing the haplogroups on either the direct paternal (Y-DNA), or direct maternal (mtDNA), these tests scan the autosomal and X chromosomes.  That's good, because that is where all of the real business is, what makes you an individual.  However, it is subject to a phenomena that we call genetic recombination (the X chromosome is a little more complicated).  This means that every generation circa 50% of both parents DNA is randomly inherited from each parent.  I said randomly.  Each generation, that randomness chops up the inherited segments smaller, and moves them around.  After about seven or eight generations, the chances of inheriting any DNA from any particular ancestral line quickly diminishes.  It becomes washed out by genetic recombination.

Therefore, not only are the autosomes subject to a randomness, and genetic recombination - they are only useful for assessing family admixture only over the past three hundred years or so.  There is arguably, DNA that has been shared between populations much further back, that we call background population admixture.  It survived, because it entered many lines, for many families, following for example, a major ancient migration event.  If this phenomena is accepted - it can only cause more problems and confusion, because it can fool results into suggesting more recent family admixture - e.g. that a great grandparent in an American family must have been Scandinavian, when in fact many Scandinavians may have settled another part of Europe, and admixed with that ancestral population, more than one thousand years ago.

DNA businesses compare segments of auDNA, against those in a number of modern day reference populations or data sets from around the world.  They look for what segments are similar to these World populations, and then try to project, what percentages of your DNA is shared or similar to these other populations.  Therefore:

  1. Your results will depend on the quality and choice of geographic boundary, allocated to any reference population data set.  A number of distinct populations of different ancestry and ethnicity may exist with in them, and cross the boundaries into other data sets.  How well are the samples chosen? Do they include urban people (that tend to have more admixture and mobility than many rural people).  Do they include descendants of migrants that merely claim a certain ancestry previous to migration?What was the criteria for sample selection?
  2. Your results might be confused by background population admixture.
  3. You are testing against modern day populations, not those of your ancestors 300 - 500 years ago.  People may well have moved around since then.  In some parts of the World, they certainly have!

It is far truer to say that your auDNA test results reflect shared DNA with modern population data sets, rather than to claim descent from them.  For example, 10% Finnish simply means that you appear to share similar DNA with a number of people that were hopefully sampled in Finland (and hopefully not just claim Finnish ancestry) - not that 10% of your ancestors came from Finland.  That is, for the above reasons, presumptuous.  It might indeed suggest some Finnish ancestry, but this is where many people go wrong, it does not prove ancestry from anywhere.

Truth

This is my main quibble.  So many testers take their autosomal (for Family/Ancestry) DNA test results to be infallible truths.  They are NOT.  White papers do not make a test and analysis system perfect and proven as accurate.  Regarding something as Science does not make it unquestionable - quite the opposite.  The fact of the matter is, if you test with different companies, different siblings, add phasing, you receive different ancestry results.  Therefore which result is true and unquestionable?

A Tool for further investigation

So what use is DNA testing for ancestry?  Actually, I would say, lots of use.  If you take the results with a pinch of salt, test with different companies, then it can help point you in a direction.  Never however take autosomal results as infallible.  Critical is to test with companies with well thought out, high quality reference data sets.  Also to test with companies that intend to progress and improve their analysis and your results.

For DNA relative matching, then sure, the companies with the best matching system, the largest match (contactable customer) databases, and with custom in the regions of the world that you hope to match with. There is also, GEDmatch.  Personally, I find it thrilling when I match through DNA, but in truth, I had more genealogical success back in the days when genealogists posted their surname interests in printed magazines and directories. 

The results of each ancestry test should be taken as a clue.  Look at the results of testers with more proven documented and known genealogies.  Learn to recognise what might be population background, as opposed to recent admixture in a family.  Investigate haplogroup DNA - it has a relative truth, although over a much longer time, and wider area.  Just be aware that your haplogroup/s represent only one or two lines of descent - your ancestry over the past few thousand years may not be well represented by a haplogroup.  Investigate everything.  Enjoy the journey.  Explore World History.

The most common misunderstanding - mtDNA

I just see so many misunderstandings on genetic genealogy and DNA test forums concerning mtDNA haplogroups, that I feel compelled to try to explain.

DNA testing businesses tend to dumb down a lot of information for their "audience".  I feel that this actually increases misunderstandings, and mtDNA haplogroups are a good example.  Rather than use the lengthy description mitochondrial DNA, or even it's shortened mtDNA, businesses describe it more frequently as Mother Line, or Maternal.  It misleads so many of their customers.  So let us put this straight:

  • A haplogroup is a  "combination of alleles at different chromosomes regions that are closely linked and that tend to be inherited together"  A series of mutations, that are inherited across generations.
  • mtDNA are a series of mutations within the DNA of mitochondria.  Mitochondria exist outside of a cell nucleus.  They have their own independent DNA, apart from the nuclear chromosomal DNA that dictates how we develop, what we are.  We all have mitchondria, in most of our cells.  They actually serve a function by processing energy.
  • As humans, we use nomenclature to group those mutations within a family tree of humanity.  My mtDNA mutations fall within Haplogroup H.
  • mtDNA cannot be passed on to future generations by males.  it is passed down to the children from the mother only.  I inherit H6a1a8 (my haplotype) from my mother, as do my brother and our sisters.  Only my sisters though will reproduce that mtDNA in their children.  My own children inherited the mtDNA of their mother, not mine.

So what does this mean in practice?

  • A Maternal / Motherline / mtDNA Haplogroup does NOT represent your biological ancestry.
  • A Maternal / Motherline / mtDNA Haplogroup does NOT even represent your mother's "half" of your biological ancestry.
  • For example, your father's mother most likely carried a different mtDNA.  Your mother's father most likely had a different mtDNA haplotype.  Only one of your sixteen great great grandparents passed down their mtDNA to you.
  • Instead, it acts pretty much as a single line genetic "marker" that can be traced only along one very narrow, single line of ancestry.  Look at the image at the top of your post.  Do you see?  Just one line of descent. It follows your mother's, mother's, mother line, and so on, all of the way back to a hypothetical "Mitochondrial Eve" 100,000 to 200,000 years ago.
  • It is not a tribe, ethnicity, or identity.  It is just the mtDNA genetic marker (Haplotype) that you inherited from your mother.
  • It is no good going onto mtDNA genetic genealogy forums and giving the names and origins of ANY direct ancestor, other than a woman (or her children) on that maternal line (mother's mother's, mother, and so on).
  • Forget surname studies.  In most western societies, and in many other's, the "family" name is inherited from the father - and follows a completely different course (Y-DNA).  Indeed, the surname of your true mtDNA ancestor changes most generations with marriage.  That is what makes this the most difficult line to trace with documentary methods.
  • Although difficult, it is the most true and secure.  Although secret or hidden adoptions can occur, the risk of non-parental events is much lower than for the strictly male line (Y-DNA).
  • Mitochondrial DNA mutates at a very slow rate.  This, along with the change in surnames most generations, can make it difficult to use successfully for genetic genealogy.  Many of the mutations are thousands of years old.  Alternatively, it makes it a valuable evidence for tracing ancient ancestry within a population.

That is all that I wanted to say.  it is a fascinating marker, but it is not representative of even 50% of your ancestry, it is not an identity, it is pretty irrelevant to surname (studies), it is inherited only down one narrow line - but all of the way back.

My earliest mtDNA ancestor with a surviving photograph.  My mother's mother's, mother's, mother (2xgreat grandmother), born Sarah Daynes in Norfolk, during 1845.  Her mtDNA would be H6a1a8.  Her mother was born Sarah Quantrill in Norfolk during 1827.  Her mother in turn was born Mary Page in Norfolk during 1791.  Her mother in turn was born Elizabeth Hardiment in Norfolk during 1751.  Her mother in turn (my 6xgreat grandmother) was Susannah Briting, who married John Hardyman in Norfolk during 1747.  If my documentary research along this line is correct, then Susannah inherited mtDNA haplotype H6a1a8 from her mother.

My Global 10 Genetic Map - and Frenchness!

My Global 10 Genetic Map coordinates:  PC1,PC2,PC3,PC4,PC5,PC6,PC7,PC8,PC9,PC10 ,0.019,0.0272,0.0002,-0.0275,-0.0055,0.0242,0.0241,-0.0033,-0.0029,0.0015

This is my position on the latest genetic map by David Wesolowski, of the Eurogenes Blog.  One point of interest that has been picked up on the Anthrogenica Forums, is my consistent closeness in ancestral results, to a Normand member!  Our Basal-rich K7 results were almost identical.  On 23andMe Ancestry Composition (spec mode), I just get a bit more French & German, while he gets just a bit more British & Irish.  We are close!

Another forum member argued though that it's my results that are skewed away from British, and towards North French.  He generated this map, plotting myself (marked as Norfolk in red), and my Norman Ancestral DNA twin Helge in yellow:

I had to point out though, that I've rarely seen other SE English with a record of local ancestry, test - and that the red circles representing British & Irish include many people with some Irish, Scottish, Welsh, Western, or Northern ancestry.  The map suggests a pull to Northern France, Belgium, and the Netherlands.

As I commented towards the end of my last post, I initially expected a pull to Denmark, Northern Germany, and perhaps to the Netherlands.  This is because so many of my 17th-20th century ancestors lived on what was the frontier of Anglo-Saxon and Danish immigration during the 4th to 11th centuries.

But instead, autosomal DNA tests for ancestry all seem to be suggesting more shared ancestry from a more southerly direction - Northern France and Belgium particularly.  Although there has so far been a dearth of local testers from local families, the POBI survey seems to find this common among the English.  We appear to be a halfway house between Old British, and the French, more than the ancestors of Anglo-Saxons and Danes.  This contradicts the historical and archaeological records.  POBI suggested that this was due to waves of unrecorded immigration from the South during late prehistory.  Others have pointed the finger at Norman and French admixture in medieval Southern Britain.  It could be both!

Can I apply for French citizenship?