Ice Age Genealogy

The MDLP K11 Modern and K13 Modern calculators were released on Gedmatch.com today.  I thought that I'd celebrate by taking the opportunity to post more on ancient origins, as suggested by personal DNA calculators.

The below calculators do sometimes conflict.  Perhaps they are a long, long way from perfect.  However, one issue is that they tend to be built to look at different populations, and a different times.  Nonetheless, I feel that they are starting to tell us something.

A major revelation to myself, is that go back, say towards the end of the last Ice Age, 13,000 years ago, and the ancestors of this present day Englishman were scattered in the most unexpected places, over a wide area.

My Y ancestor at the time was most likely an ibex hunter in the area of Iran and Iraq, possibly in the Zagros mountains.  My mitochondrial ancestor, was most likely a woman in a band of hunter-gatherers in Central or Western Asia.

I most likely at that time, had mammoth hunting ancestors in Siberia (Ancient North Eurasian).  I had hunter-forager ancestors in the Middle East or Arabia (Basal Eurasian).  I may have had ancestors in the Caucasus themselves.  I may have even had some hunter-gatherer ancestors in Southern Europe.

My Ancient Ancestors 13,000 years ago, were most likely, scattered in small hunter-gatherer bands, from Southern Europe, across to Central Asia, and from Siberia, down to Arabia.  That's the Late Ice Age ancestry of this Englishman.  I'm a Eurasian.  The idea that my Upper Palaeolithic ancestors were cave men in the British Isles, or even in North-West Europe, is an illusion.

The other factor is that, perhaps in slightly different percentages - all Western Eurasians today share the very same Ice Age ancestors - English, Iranian, French, German, Pakistani, Spanish, Armenian, Russian, or Norwegian.  We are all pretty closely related cousins in one post Ice Age family, that has literally exploded with agriculture, then industrialisation.

Neanderthal Ancestry

23andMe V4 chip

Neanderthal ancestry 2.9% DNA (82nd percentile)  23andMe average European tester is 2.7%

WeGene analysis of above 23andMe raw data

3.325% Neanderthal proportion of more than 81.94% of the users WeGene (Chinese based DNA service).

Upper Palaeolithic and Mesolithic Hunter-Gatherer ancestry

My Y line as we have seen, was most likely in the area of modern day Iran or Iraq, perhaps in the Euphrates and Tigris valleys, or perhaps in the Zagros Mountains, hunting animals such as the Ibex.  My mt-DNA line was most likely in a hunter-gatherer band somewhere in Asia.  Perhaps Central Asia.  What about my other Ice Age ancestors?

David Wesolowski's K7 Basal-rich test

Villabruna-related

The Villabruna cluster represents the DNA found in 13 individuals in Europe from after 14,000 years ago.  They were Late Ice Age hunter-gatherers.  They appear to have links with the Near East.  The current thought is that they replaced earlier groups of hunter-gatherers in Europe.  The DNA of people in the Middle East and Europe pulled together at this time, and they may represent an expansion from the South-East.  Much of the Aegean Sea would have been dry, with low sea levels (glaciation), so the migration may have been easy.  It is believed that they had dark skin, and blue eyes.  They were possibly, the last hunter-gatherers of Europe and the Middle East.  They may have contributed to our DNA both through or either, later Asian or European admixtures.

David gives the English average as 56.7%.  My result is 57.1%

Basal-rich

The Basal Eurasians are a hypothetical "ghost" population derived from DNA studies.  It is suggested that they splintered from other modern humans 45,000 years ago, presumably outside of Africa, somewhere around the Middle East.  They significantly contributed DNA to the Early Neolithic Farmers of the Fertile Crescent and Anatolia, and consequently, on to all of us modern West Eurasians.  

 David gives the English average as 26.5%.  My result is 28.8%

Ancient North Eurasian

Another Ice Age hunter-gatherer "Ghost" population, but this one has been associated with human remains and an Upper Palaeolithic culture (Mal'ta-Buret') at Lake Baikal, Siberia.  We know that it significantly contributes to modern West Eurasians, through earlier admixture on the Eurasian Steppes.  Copper Age pastoralists then carried it westwards into Europe with their later expansion.

David gives the English average as 16.6%.  My result is 14.0%

Neolithic and Bronze Age mix ancestry

My Y line at this time as we have seen, may well been Early Neolithic Farmers in the Fertile Crescent of Mesopotamia.  My mtDNA line would have most likely have been women of the Yamnaya culture on the Eurasian Steppes, in Copper Age pastoralist tribes.

Global 10 Test

The recent Global 10 test, run by my friend Helgenes50 of the Anthrogenica board, resulted in:

• 55% Baalberge_MN (European Middle Neolithic)
• 38% Yamna_Samara (Eurasian Steppe Pastoralist)
• 7% Loschbour:Loschbour (Late Eurasian hunter-gatherer)

That is 55% European Neolithic Farmer, 38% Yamnaya Steppe Pastoralist, and 7% European hunter-gatherer.

Alternatively, the FT-DNA test, although many in the population genetics community feel that it is unreliable:

FT-DNA My Ancient Origins

• 9% Metal Age Invader
• 47% Farmer
• 44% Hunter-Gatherer
• 0% Non European

GEDMatch Ancient Calculators

• 31% Neolithic (modeled on genomes of first neolithic farmers of Anatolia)
• 25% Northeast European (ancestry in North-Eastern Europe based on older type of ancestry (WHG, west European Hunter-Gatherer)
• 22% Steppe (sourced from ancient genome of European Bronze Age pastoralists)
• 22% Caucasian (derived from genomes of mesolithic Caucasian Hunter-gatherers)

My Eurasia K9 ASI Oracle:

• 39% Western Hunter-Gatherer
• 27% Early Neolithic Farmer
• 15% Eastern Hunter-Gatherer
• 12% Caucasus Hunter-Gatherer
• 7% SW Asian
• 1% Siberian East Asian

My MDLP Modern K11 Oracle:

Admix Results (sorted):

#	Population	Percent
1	Neolithic	37.33
2	WHG	33.26
3	EHG	23.19
4	Iran-Mesolithic	3.25
5	Basal	2.66

Using 1 population approximation:
1 British_Celtic @ 6.948432
2 Bell_Beaker_Germany @ 8.143357
3 Alberstedt_LN @ 8.426399
4 British_IronAge @ 9.027687
5 Halberstadt_LBA @ 10.273615
6 Bell_Beaker_Czech @ 12.190828
7 Hungary_BA @ 12.297826
8 Nordic_MN_B @ 12.959966
9 British_AngloSaxon @ 12.993559
10 Nordic_BA @ 13.170285

Above image by Marta D. Costa, Joana B. Pereira, Maria Pala, Verónica Fernandes, Anna Olivieri, Alessandro Achilli, Ugo A. Perego, Sergei Rychkov, Oksana Naumova, Jiři Hatina, Scott R. Woodward, Ken Khong Eng, Vincent Macaulay, Martin Carr, Pedro Soares, Luísa Pereira & Martin B. Richards [CC BY 3.0], via Wikimedia Commons

I'm gathering here, data on H6a1a, in preparation for my expected FT-DNA mtFull Sequence test results later this month.  23andMe has previously tested me to H6a1.  The James Lick and Wegene analysis services both predict me to be H6a1a8. What will FT-DNA say?

A collection of ancient references for mitochondrial DNA haplogroup H6 so far (2017-01-02) harvested from Ancestral Journeys

Palaeolithic Western Eurasian aDNA

No mtDNA H6 so far found.  Mainly U

Mesolithic Western Eurasian aDNA

No mtDNA H6 so far found.  Mainly U.  Other H found in Russia, Georgia, and Sweden.

Near Eastern Neolithic aDNA

No mtDNA H6 so far found.  Some H.

European Neolithic aDNA

No mtDNA H6 so far found.  Many other H.

Copper and Bronze Age aDNA

• H6.  Israel Wadi el Makkukh. 4240-4065 BC
• H6a1b.  Yamnaya.  Russia Kutuluk River, Samara. 3300-2700 BC
• H6a2.  Potavka.  Russia Kutuluk River, Samara.2867-2484 BC
• H6a1a.  Corded Ware.  Germany.  Esperstedt.  2465-2395 BC
• H6a1b.  Okunevo.  Russia.  Verkhni Askiz.  2201-2036 BC
• H6a1b3.  Unetice.  Germany.  Leau.  2200-1550 BC
• H6a1a.  Srubnaya.  Russia.  Spiridonovka, Samara River.  1913-1629 BC

Iron Age aDNA

• H6a1a8. On yFULL id: 16413 and 16495. Haddingtonshire East Lothian, Scotland dated 1914 ybp and 2047 ybp.

Ancient Roman aDNA

• H6a1a.  Romano-British.  England.  York.  100-400 AD
• H6a1b2. Romano-British.  England.  York. 100-400 AD.

Medieval and later European and Western Asian aDNA

• H6a1b1.  Lombard.  Italy.  600-800 AD
• H6.  Viking.  Oppland, Norway.
• H6a1a.  Magyar.  Karos-Eperjesszög, Hungary. 800-850 AD
• H6a1b. Magyar.  Karos-Eperjesszög, Hungary. 900-950 AD
• H6a1a.  Medieval English.  East Smithfield, London, England.  1347–1351 AD.

Discussion

Above image by User:Dbachmann [GFDL or CC-BY-SA-3.0], via Wikimedia Commons

Although this is based on limited samples (albeit rapidly growing samples over the past year), there is of mt haplogroup H spread across Western Eurasia, certainly by the Mesolithic period.  Today, it is the most common haplogroup in Europe, found in 41% of Europeans.

H6 however, is less certain.  It has been suggested that it is more frequent in Eastern Europe, and the Caucasus.  The above ancient DNA references, collected so far, might suggest that H6a1 arrived into Europe with the Yamnaya expansion off of the Eurasian Steppes, carried by Steppe pastoralists migrating westwards, during the Copper Age / Early Bronze Age.  H6a1a itself, first appears in the Corded Ware Culture, which has recently been recognised as a fusion culture, that developed in Eastern / Central Europe, as a result of the arrival of the Steppe pastoralists.

The samples above suggest that H6a1a was present in Britain previous to the Anglo-Saxon period, and was also present in Medieval London.

H6a1a Today

Harvested from the FT-DNA MT-DNA H6 Haplogroup Project (2017-01-02).  These samples are most likely affected by a bias in sampling, to people of European heritage.

H6a1a - T11253C.  Britain, Ireland, Netherlands, Central Europe, Italy, Iberia, Balkans.  A cluster in SW Britain probably caused by sampling bias.

• H6a1a* - G16526A.  Czech Rep.  Poland.
• H6a1a* - T3548C.  Sweden, and Sicily.
• H6a1a2a - C41T, G16482A.  Lithuania
• H6a1a2a* - A297G, A14970G.  England
• H6a1a3 - T5785C.  England (Norfolk), Germany, Poland, and Ukraine.
• H6a1a3* - T7094C.  Finland.
• H6a1a3a - A827G.  Scotland
• H6a1a4 - T10237C.  Norway, Poland, England, and Ukraine.
• H6a1a5 - C10936T.  Very Eastern Europe - Russia, Belarus, Ukraine, Moldovia, and Romania.
• H6a1a5* - T5302C.  Russia.
• H6a1a6 - A288G.  Poland.
• H6a1a8a - T6185C, G16145A.  Wales.

Haplogroup.org.

The Haplogroup.org website proposes the following dates:

H6a1

Age: 8,669.7 ± 1,905.2; CI=95% (Behar et al., 2012b)

Origin: Undetermined
Mutations: A4727G
Origin: Undetermined
Mutations: T11253C
Origin: Undetermined
Mutations: T16298C

2017-01-04 UPDATE

Behar et al., 2012b, dates H6a1 to 8,669.7 ± 1,905.2; CI=95%, H6a1a to 7,139.5 ± 1,993.2; CI=95%, and H6a1a8 to 2,023.7 ± 1,898.9; CI=95% So I see H6a1a8 as pretty young, most likely Bronze Age or Iron Age. I'm curious as to when it distributed through Europe, and when it likely entered the British Isles. My mt-line has been in Norfolk, England for at least 290 years, when my earliest mt ancestor, Susannah Briting was born. She married my ancestor at Bunwell, Norfolk in 1747. I'd say that the probability is that it had been here in East Anglia long before that paper trail. So when did it likely move here?

23andMe had previously tested me to H6a1. Both the James Lick mthap analyser, and WeGene, looking at the 23andMe raw data, took that to H6a1a8. They were correct:

The FT-DNA mtFull Sequence confirmed my mt haplogroup to be H6a1a8.

My HRV1 mutations are: A16129G, T16187C, C16189T, T16223C, G16230A, T16278C, T16298C, C16311T, T16362C, A16482G, C16519T
My HRV2 mutations are: G73A, C146T, C152T, C195T, T239C, A247G, 522.1A, 522.2C, 309.1C, 309.2C, 315.1C
Extra mutations are: 309.1C309.2C315.1C522.1A522.2CC16519T

HRV1 matches are:

England 3
Hungary 1
Ireland 4
UK 1
USA 1

HRV1 + 2 matches are:

England 2
Hungary 1
Ireland 3

I have a "HRV1, HRV2, and Coding Region" exact match: Ireland 2

On Matches I get four GD0, but all appear USA/Aus, etc. I have emailed them. Three replied. No paper or geographical correlations. They can't follow their mt lines previous to emigration. They could come from anywhere in Europe, although English surnames keep dominating on their maternal sides.

On Matches map, a little more hope:



I've joined the H6 project, but being a development of the pretty massive mt hg H Project, it is drowned in submissions, with a long waiting queue of "ungrouped". The only other H6a1a8 on the results is USA. There is a H6a1a8a ungrouped as private located as "Finland", and another H6a1a8a located to Wales.

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